Development of antipeptide enzyme-linked immunosorbent assay for determination of gelatin in confectionery products

被引：25
作者：
Tukiran, Nur Azira [1 ,2 ]
Ismail, Amin [1 ,3 ]
Mustafa, Shuhaimi [1 ,4 ]
Hamid, Muhajir [4 ]
机构：
[1] Univ Putra Malaysia, Halal Prod Res Inst, Lab Halal Sci Res, Serdang 43400, Selangor, Malaysia
[2] IIUM, Int Inst Halal Res & Training INHART, Kuala Lumpur 50728, Malaysia
[3] Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Nutr & Dietet, Serdang 43400, Selangor, Malaysia
[4] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Microbiol, Serdang 43400, Selangor, Malaysia
来源：
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2016年 / 51卷 / 01期
关键词：
Collagen alpha 2 (I) chain; enzyme-linked immunosorbent assay; gelatin; polyclonal antibody; SDS-PAGE; PROTEOMIC ANALYSIS; AMINO-ACIDS; BOVINE; DIFFERENTIATION; EXPRESSION; PROTEIN; EXTRACTION; MEAT;
D O I：
10.1111/ijfs.12971
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The gelatin sources have become a controversial issue with regard to religious and health concern. Thus, the aims of this study were to develop and evaluate the efficiency of polyclonal antibodies against peptide immunogen of collagen alpha 2 (I) chain for determination of gelatin sources in confectionery products by competitive indirect enzyme-linked immunosorbent assay (ELISA). Collagen alpha 2 (I) chain protein showed resistance against heat treatment and detectable in certain commercial products when analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The established ELISA exhibited low cross-reactivity to fish and chicken gelatin. The IC50 value was 0.39 mu g mL(-1), and the limit of detection (IC10) was 0.05 mu g mL(-1) . There were no false-positive results from forty-eight commercially processed products. The present method is useful for determination of gelatin in confectionery products.
引用
收藏
页码：54 / 60
页数：7
相关论文
共 26 条
[1]  
Aina M. A., 2013, International Food Research Journal, V20, P1395
[2]  
AOAC Int, 2012, VAL PROC QUANT FOOD
[3]   Validation of a reverse-phase high-performance liquid chromatography method for the determination of amino acids in gelatins by application of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate reagent [J].
Azilawati, M. I. ;
Hashim, D. M. ;
Jamilah, B. ;
Amin, I. .
JOURNAL OF CHROMATOGRAPHY A, 2014, 1353 :49-56
[4]   Use of principal component analysis for differentiation of gelatine sources based on polypeptide molecular weights [J].
Azira, T. Nur ;
Man, Y. B. Che ;
Hafidz, R. N. Raja Mohd ;
Aina, M. A. ;
Amin, I. .
FOOD CHEMISTRY, 2014, 151 :286-292
[5]   Extraction and functional properties of gelatin from the skin of cuttlefish (Sepia officinalis) using smooth hound crude acid protease-aided process [J].
Balti, Rafik ;
Jridi, Mourad ;
Sila, Assaad ;
Souissi, Nabil ;
Nedjar-Arroume, Naima ;
Guillochon, Didier ;
Nasri, Moncef .
FOOD HYDROCOLLOIDS, 2011, 25 (05) :943-950
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]   Biotin-streptavidin enzyme-linked immunosorbent assay for detecting Tetrabromobisphenol A in electronic waste [J].
Bu, Dan ;
Zhuang, Huisheng ;
Zhou, Xinchu ;
Yang, Guangxin .
TALANTA, 2014, 120 :40-46
[8]   Proteomic analysis reveals that treatment with tocotrienols reverses the effect of H2O2 exposure on peroxiredoxin expression in human lymphocytes from young and old individuals [J].
Dahlan, Hasnizawati Mohamed ;
Karsani, Saiful Anuar ;
Rahman, Mariati Abdul ;
Hamid, Noor Aini Abdul ;
Top, A. Gapor Mat ;
Ngah, Wan Zurinah Wan .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2012, 23 (07) :741-751
[9]   A Reliable Enzyme Linked Immunosorbent Assay for the Determination of Bovine and Porcine Gelatin in Processed Foods [J].
Doi, Hirotoshi ;
Watanabe, Eriko ;
Shibata, Haruki ;
Tanabe, Soichi .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (05) :1721-1726
[10]   Detection of hazelnuts and almonds using commercial ELISA test kits [J].
Garber, Eric A. E. ;
Perry, Jesse .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 396 (05) :1939-1945
123