Single-Molecule Reconstruction of Oligonucleotide Secondary Structure by Atomic Force Microscopy

被引：93

作者：

Pyne, Alice ^{[1
]}

Thompson, Ruth ^{[1
]}

Leung, Carl ^{[1
]}

Roy, Debdulal ^{[2
]}

Hoogenboom, Bart W. ^{[1
]}

机构：

[1] UCL, London Ctr Nanotechnol, London WC1H 0AH, England

[2] Natl Phys Lab, Teddington TW11 0LW, Middx, England

来源：

SMALL | 2014年 / 10卷 / 16期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

DNA; VISUALIZATION;

D O I：

10.1002/smll.201400265

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Based on soft-touch atomic force microscopy, a method is described to reconstruct the secondary structure of single extended biomolecules, without the need for crystallization. The method is tested by accurately reproducing the dimensions of the B-DNA crystal structure. Importantly, intramolecular variations in groove depth of the DNA double helix are resolved, which would be inaccessible for methods that rely on ensemble-averaging. © 2014 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：3257 / 3261

页数：5

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