Autofocusing and edge detection schemes in cell volume measurements with quantitative phase microscopy

被引：57

作者：

Lee, Seungrag ^{[1
]}

Lee, Ji Yong ^{[1
]}

Yang, Wenzhong ^{[1
]}

Kim, Dug Young ^{[1
]}

机构：

[1] Gwangju Inst Sci & Technol, Dept Informat & Commun, Kwangju 500712, South Korea

来源：

OPTICS EXPRESS | 2009年 / 17卷 / 08期

关键词：

DIFFRACTION PHASE; REFRACTIVE-INDEX; LIVING CELLS; DYNAMICS;

D O I：

10.1364/OE.17.006476

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have proposed and demonstrated a very sensitive volume measurement scheme for a live cell with a quantitative phase microscopy (QPM) utilizing auto-focusing and numerical edge detection schemes. An auto-focusing technique with two different focus measures is applied to find the focus dependent errors in our live cell volume measurement system. The volume of a polystyrene bead sample with 3 mu m diameter has been measured for the validity test of our proposed method. We have shown that a small displacement of an object from its focusing position can cause a large volume error. A numerical edge detection technique is also used to accurately resolve the boundary between a cell and its suspension medium. We have applied this method to effectively suppress errors by the surrounding medium of a single red blood cell (RBC). (C) 2009 Optical Society of America

引用

页码：6476 / 6486

页数：11

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