Pulsed-field gel electrophoresis indicates genotypic heterogeneity among Campylobacter upsaliensis strains

被引：13
作者：
Bourke, B
Sherman, PM
Woodward, D
Lior, H
Chan, VL
机构：
[1] HOSP SICK CHILDREN,RES INST,DIV GASTROENTEROL,TORONTO,ON M5G 1X8,CANADA
[2] UNIV TORONTO,DEPT PEDIAT,TORONTO,ON M5S 1A8,CANADA
[3] UNIV TORONTO,DEPT MICROBIOL,TORONTO,ON M5S 1A8,CANADA
[4] LAB CTR DIS CONTROL,OTTAWA,ON K1A 0L2,CANADA
来源：
FEMS MICROBIOLOGY LETTERS | 1996年 / 143卷 / 01期
关键词：
Campylobacter; epidemiology; chromosome;
D O I：
10.1016/0378-1097(96)00290-X
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
To determine the genomic relatedness among a selection of animal and human Campylobacter upsaliensis isolates, macrorestriction profiles were generated for 20 C. upsaliensis strains, among 7 serogroups, using pulsed-field gel electrophoresis (PFGE). XhoI, SalI and SacII restriction enzyme profiles indicated genomic heterogeneity among strains. Using XhoI and SacII restriction enzyme digestion, genomic similarities between some pairs of strains were Lier serogroup specific. The genomic sizes of these isolates varied from 1.74 to 2.09 Mb. These results demonstrate molecular heterogeneity of this species similar to that found among Helicobacter pylori isolates. Among C. upsaliensis strains, PFGE is highly discriminatory and should prove a useful molecular typing method for epidemiological purposes.
引用
收藏
页码：57 / 61
页数：5
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