Analysis of PTP1B sumoylation

被引：10
|
作者：
Saha, Sayanti [1 ]
Chernoff, Jonathan [1 ]
机构：
[1] Fox Chase Canc Ctr, Canc Biol Program, Philadelphia, PA 19111 USA
来源：
METHODS | 2014年 / 65卷 / 02期
关键词：
Signal transduction; Protein tyrosine phosphatases; Phosphorylation; Posttranslational modification; Sumoylation; TYROSINE-PHOSPHATASE; 1B; PROTEIN SUMOYLATION; SUMO MODIFICATION; ESCHERICHIA-COLI; UBC9; LOCALIZES; RECEPTOR;
D O I：
10.1016/j.ymeth.2013.09.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Signaling enzymes are typically regulated by a variety of post-translational modifications. One such enzyme, protein tyrosine phosphatase (PTP) 1B, which plays an important role in metabolic and growth signaling, has recently been shown to be inhibited by posttranslational modifications by ubiquitin-related proteins of the SUMO family. We have adapted several approaches to analyzing the sites and effects of sumoylation of PTP1B in cells and in vitro. The principal outline of the assays should be applicable to other enzymes as well. (C) 2014 Published by Elsevier Inc.
引用
收藏
页码：201 / 206
页数：6
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