Detection of Mycobacterium tuberculosis complex with Real Time PCR:: Comparison of different primer-probe sets based on the IS6110 element

被引：46

作者：

Savelkoul, Paul H. M. ^{[1
]}

Catsburg, Arnold

Mulder, Sije

Oostendorp, Ludo

Schirm, Jurjen

Wilke, Hans

van der Zanden, Adri G. M.

Noordhoek, Gerda T.

机构：

[1] Vrije Univ Amsterdam Med Ctr, Dept Med Microbiol & Infect Control, Amsterdam, Netherlands

[2] Publ Hlth Lab Friesland, Leeuwarden, Netherlands

[3] Lab Infect Dis, Groningen, Netherlands

[4] Reg Publ Hlth Lab, Enschede, Netherlands

[5] Gelre Hosp, Dept Microbiol, Apeldoorn, Netherlands

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 66卷 / 01期

关键词：

Mycobacterium tuberculosis; Real Time PCR; IS6110;

D O I：

10.1016/j.mimet.2005.12.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The sensitivity and specificity to detect Mycobacterium tuberculosis complex of four Real Time PCR primer-probe sets was compared. Three sets targeted nearly the same location on the IS6110 sequence and set 4 targeted a location 200 bp downstream on IS6110. Real Time PCR's with sets 1, 2 and 3 were carried out with co-amplification of a modified target as an internal amplification control. By testing identical DNA samples it was shown that small changes in primer and probe sequences result in differences in the performance of the assays, regarding analytical sensitivity and specificity. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：177 / 180

页数：4

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