Purification and Characterization of a Tuliposide-Converting Enzyme from Bulbs of Tulipa gesneriana

被引：17

作者：

Kato, Yasuo ^{[1
]}

Shoji, Kazuaki ^{[2
]}

Ubukata, Makoto ^{[3
]}

Shigetomi, Kengo ^{[3
]}

Sato, Yukio ^{[1
]}

Nakajima, Noriyuki ^{[1
]}

Ogita, Shinjiro ^{[1
]}

机构：

[1] Toyama Prefectural Univ, Fac Engn, Biotechnol Res Ctr, Toyama 9390398, Japan

[2] Toyama Prefectural Agr Forestry & Fisheries Res C, Toyama 9398153, Japan

[3] Hokkaido Univ, Res Fac Agr, Div Appl Biosci, Kita Ku, Sapporo, Hokkaido 0608589, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 08期

关键词：

tulip; tuliposide; tulipalin; enzyme purification; Tulipa gesneriana; BETA-GLUCOSIDASES; DEFENSE; GENE;

D O I：

10.1271/bbb.90226

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana. The enzyme appeared to be a dimer, the relative molecular mass (Mr) of each subunit being 34,900; it had maximum activity and stability at neutral pH and moderate temperature. The enzyme preferentially acted on such glucose esters as 6-tuliposides, and to a lesser extent on p-nitrophenylacetate.

引用

页码：1895 / 1897

页数：3

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