Purification and Characterization of a Tuliposide-Converting Enzyme from Bulbs of Tulipa gesneriana

被引：17
|
作者：
Kato, Yasuo [1 ]
Shoji, Kazuaki [2 ]
Ubukata, Makoto [3 ]
Shigetomi, Kengo [3 ]
Sato, Yukio [1 ]
Nakajima, Noriyuki [1 ]
Ogita, Shinjiro [1 ]
机构：
[1] Toyama Prefectural Univ, Fac Engn, Biotechnol Res Ctr, Toyama 9390398, Japan
[2] Toyama Prefectural Agr Forestry & Fisheries Res C, Toyama 9398153, Japan
[3] Hokkaido Univ, Res Fac Agr, Div Appl Biosci, Kita Ku, Sapporo, Hokkaido 0608589, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 08期
关键词：
tulip; tuliposide; tulipalin; enzyme purification; Tulipa gesneriana; BETA-GLUCOSIDASES; DEFENSE; GENE;
D O I：
10.1271/bbb.90226
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana. The enzyme appeared to be a dimer, the relative molecular mass (Mr) of each subunit being 34,900; it had maximum activity and stability at neutral pH and moderate temperature. The enzyme preferentially acted on such glucose esters as 6-tuliposides, and to a lesser extent on p-nitrophenylacetate.
引用
收藏
页码：1895 / 1897
页数：3
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