Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

被引：73

作者：

Sandberg, Malena E. ^{[1
]}

Schellmann, Denise ^{[2
]}

Brunhofer, Gerda ^{[2
]}

Erker, Thomas ^{[2
]}

Busygin, Igor ^{[3
]}

Leino, Reko ^{[3
]}

Vuorela, Pia M. ^{[1
]}

Fallarero, Adyary ^{[1
]}

机构：

[1] Abo Akad Univ, Dept Biochem & Pharm, Div Pharm, FI-20520 Turku, Finland

[2] Univ Vienna, Dept Med Chem, Vienna, Austria

[3] Abo Akad Univ, Organ Chem Lab, FI-20520 Turku, Finland

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 78卷 / 01期

基金：

芬兰科学院;

关键词：

Biofilms; Resazurin; Screening; Staphylococcus aureus; DISINFECTION;

D O I：

10.1016/j.mimet.2009.04.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Staining of Staphylococcus aureus biofilms with 20 mu M resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：104 / 106

页数：3

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