End-Group Analysis of Bacterially Produced Poly(3-hydroxybutyrate): Discovery of Succinate as the Polymerization Starter

被引：18

作者：

Yamanaka, Kenji ^{[1
]}

Kimura, Yoshiharu ^{[1
]}

Aoki, Takashi ^{[1
]}

Kudo, Toshiji ^{[2
]}

机构：

[1] Kyoto Inst Technol, Dept Biomol Engn, Sakyo Ku, Kyoto 6068585, Japan

[2] Bruker Dalton KK, Sales & Tech Support Ctr, Kanagawa Ku, Kanagawa 2210022, Japan

来源：

MACROMOLECULES | 2009年 / 42卷 / 12期

关键词：

MULTISTAGE MASS-SPECTROMETRY; ALCALIGENES-EUTROPHUS; RALSTONIA-EUTROPHA; SEQUENCE DISTRIBUTION; BETA-HYDROXYBUTYRATE; MOLECULAR-WEIGHT; PHB SYNTHASE; MECHANISM; DEGRADATION; POLYESTERS;

D O I：

10.1021/ma900427z

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The end groups of bacterial poly(3-hydroxybutyrate) (PHB) were analyzed by H-1 and P-31 NMR spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A PHB component having a molecular weight around 200 000 Da was broken down into low-molecular-weight oligomers by acid-catalyzed partial hydrolysis. MALDI-TOF MS showed that one series of fragments contain a common terminal group that originated from the primer used for initiating in vivo polymerization. Analysis of the fragments proved that the terminal group is succinate. On the basis of this result, a plausible mechanism is proposed for enzymatic polymerization in microbial PHB synthesis.

引用

页码：4038 / 4046

页数：9

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