Morphine determination by HPLC with improved chemiluminescence detection using a conventional silica based column

被引：14
作者：
Amiott, E
Andrews, ARJ
机构：
[1] Department of Chemistry, Ohio University, Athens
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1997年 / 20卷 / 02期
关键词：
D O I：
10.1080/10826079708010655
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new high performance liquid chromatography-chemiluminescence analysis system for morphine is described. This system has an on-column limit of detection of 20 pg for morphine, allowing morphine determination down to 1 ng/mL, with a 20 mu L injection size, without any concentration during sample preparation. Simultaneous determination of monoacetylmorphine to 15 ng/mL (300 pg on-column) is also achieved. The internal standard for both compounds is nalorphine and total analysis time is under 5 minutes.
引用
收藏
页码：311 / 325
页数：15
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