Cross-Linked Elastin-like Polypeptide Membranes as a Model for Medial Arterial Calcification

被引：20
|
作者：
Gourgas, Ophelie [1 ]
Muiznieks, Lisa D. [2 ]
Bello, Dainelys Guadarrama [4 ]
Nanci, Antonio [4 ,5 ]
Sharpe, Simon [2 ,3 ]
Cerruti, Marta [1 ]
机构：
[1] McGill Univ, Dept Min & Mat Engn, Montreal, PQ H3A 0C5, Canada
[2] Hosp Sick Children, Mol Med, Toronto, ON M5G 0A4, Canada
[3] Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada
[4] Univ Montreal, Fac Dent Med, Dept Stomatol, Montreal, PQ H3C 3J7, Canada
[5] Univ Montreal, Fac Med, Dept Biochem & Mol Med, Montreal, PQ H3C 3J7, Canada
来源：
BIOMACROMOLECULES | 2019年 / 20卷 / 07期
基金：
加拿大创新基金会; 加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词：
AMORPHOUS CALCIUM-PHOSPHATE; TRANSFORM INFRARED-SPECTROSCOPY; RAY-ABSORPTION SPECTROSCOPY; EXPRESSED HUMAN ELASTIN; VASCULAR CALCIFICATION; EXTRACELLULAR-MATRIX; BONE REGENERATION; IN-VIVO; SELF-AGGREGATION; MINERALIZATION;
D O I：
10.1021/acs.biomac.9b00417
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Calcium phosphate minerals deposit on the elastin-rich medial layers of arteries in the majority of seniors, diabetic, and chronic kidney disease patients, causing severe cardiovascular complications. There is no cure for medial calcification, and the mechanism of mineral formation on elastin layers is unknown. Here we propose cross-linked elastin-like polypeptide membranes as models to study medial calcification. Calcium phosphates deposit first on fibers and filaments and then spread to globular structures present in the membranes. Mineral phase evolution analyzed by near-edge X-ray spectroscopy matches that previously observed in a mouse model of medial calcification, showing that this simple system captures some of the key in vivo findings. This work shows how minerals form and evolve upon nucleation on elastin and provides an in vitro model that can be tuned to study hypotheses related to arterial calcification mechanisms and test drugs to stop or revert mineralization.
引用
收藏
页码：2625 / 2636
页数：12
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