Oxidative stress and antioxidant defenses in two green microalgae exposed to copper

被引：236

作者：

Sabatini, Sebastian E. ^{[1
,2
]}

Juarez, Angela B. ^{[1
,3
]}

Eppis, Maria R. ^{[4
]}

Bianchi, Laura ^{[4
]}

Luquet, Carlos M. ^{[5
]}

del Carmen Rios de Molina, Maria ^{[1
,2
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Biol, RA-1428 Buenos Aires, DF, Argentina

[2] Consejo Nacl Invest Cient & Tecn, RA-1917 Buenos Aires, DF, Argentina

[3] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, RA-1428 Buenos Aires, DF, Argentina

[4] CAC Comis Nacl Energia Atom, UA Combustibles Nucl, RA-1650 Buenos Aires, DF, Argentina

[5] Univ Nacl Comahue, CONICET, INIBIOMA, Lan Ecotoxicol Acuat, RA-8370 Neuquen, Argentina

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2009年 / 72卷 / 04期

关键词：

Scenedesmus vacuolatus; Chlorella kessleri; Copper content; Oxidative stress; Total reflection X-ray fluorescence (TXRF); Metabolic damage; Lipid peroxidation; Reduced glutathione; Superoxide dismutase; Catalase; METAL-SUBSTITUTED CHLOROPHYLLS; DIATOM DITYLUM-BRIGHTWELLII; LIPID-PEROXIDATION; CHLORELLA-VULGARIS; MARINE-ALGAE; HEAVY-METALS; TOXICITY; BIOACCUMULATION; BIOSYNTHESIS; GLUTATHIONE;

D O I：

10.1016/j.ecoenv.2009.01.003

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The aim of this work was to assess the effects of 1 week copper exposure (6.2,108, 210 and 414 mu M) on Scenedesmus vacuolatus and Chlorella kessleri. The strains showed different susceptibility to copper. Copper content was determined in both strains by total X-ray reflection fluorescence analysis (TXRF). In S. vacuolatus, the increase of medium copper concentration induced an augmentation of protein and MDA content, and a significant decrease in the chlorophyll a/chlorophyll b ratio. S. vacuolatus showed a significant increase of catalase activity in 210 and 414 mu M of copper, and a significant increment of SOD activity and GSH content only in 414 mu M of copper. On the contrary, C. kessleri did not show significant differences in these parameters between 6.2 and 108 mu M of copper. Increased copper in the environment evokes oxidative stress and an increase in the antioxidant defenses of S. vacuolatus. (c) 2009 Elsevier Inc. All rights reserved.

引用

页码：1200 / 1206

页数：7

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