Prion protein allele A136H154Q171 is associated with high susceptibility to scrapie in purebred and crossbred German Merinoland sheep

被引:65
作者
Lühken, G
Buschmann, A
Groschup, MH
Erhardt, G
机构
[1] Univ Giessen, Dept Anim Breeding & Genet, D-35390 Giessen, Germany
[2] Fed Res Ctr Virus Dis Anim, Inst Novel & Emerging Infect Dis, Greifswald, Germany
关键词
D O I
10.1007/s00705-004-0303-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Prion protein (PrP) genotypes were determined in eight sheep that have been tested positive for atypical scrapie from purebred or crossbred Merinoland sheep flocks in Germany and compared with the PrP genotypes of their flock mates. Two restriction fragment length polymorphism (RFLP) analyses were developed to determine all PRNP haplotypes occurring by variations at codons 136, 154 and 171. At least one copy of the A(136)H(154)Q(171) (AHQ) allele was found in all scrapie-positive sheep while the frequency of AHQ varied from over 23% to less than 3% in the whole flocks. There was a significant association between PrP genotype and a positive scrapie diagnosis over all flocks, suggesting a high scrapie susceptibility of PrP genotypes including the AHQ allele, at least in sheep of Merinoland type. These results argue that sheep with the AHQ allele are not generally less susceptible to scrapie and support the hypothesis that the influence of this allele on scrapie susceptibility may vary from flock to flock depending on genetic and/or epidemiological factors. This has to be considered when strategies for the eradication of scrapie in sheep are based on PrP genotypes.
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页码:1571 / 1580
页数:10
相关论文
共 21 条
[1]   The signature of scrapie: differences in the PrP genotype profile of scrapie-affected and scrapie-free UK sheep flocks [J].
Baylis, M ;
Houston, F ;
Goldmann, W ;
Hunter, N ;
McLean, AR .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2000, 267 (1457) :2029-2035
[2]   Cases of scrapie with unusual features in Norway and designation of a new type, Nor98 [J].
Benestad, SL ;
Sarradin, P ;
Thu, B ;
Schönheit, J ;
Tranulis, MA ;
Bratberg, B .
VETERINARY RECORD, 2003, 153 (07) :202-+
[3]   PrP genotype contributes to determining survival times of sheep with natural scrapie [J].
Bossers, A ;
Schreuder, BEC ;
Muileman, IH ;
Belt, PBGM ;
Smits, MA .
JOURNAL OF GENERAL VIROLOGY, 1996, 77 :2669-2673
[4]  
BUSCHMANN A, 2003, IN PRESS J VIROL MET
[5]  
Dawson M, 1998, VET REC, V142, P623
[6]   Multiplicative genetic effects in scrapie disease susceptibility [J].
Dubois, MA ;
Sabatier, P ;
Durand, B ;
Calavas, D ;
Ducrot, C ;
Chalvet-Monfray, K .
COMPTES RENDUS BIOLOGIES, 2002, 325 (05) :565-570
[7]   Genetic susceptibility and transmission factors in scrapie: detailed analysis of an epidemic in a closed flock of Romanov [J].
Elsen, JM ;
Amigues, Y ;
Schelcher, F ;
Ducrocq, V ;
Andreoletti, O ;
Eychenne, F ;
Khang, JVT ;
Poivey, JP ;
Lantier, F ;
Laplanche, JL .
ARCHIVES OF VIROLOGY, 1999, 144 (03) :431-445
[8]   MODIFICATION OF ENZYMATICALLY AMPLIFIED DNA FOR THE DETECTION OF POINT MUTATIONS [J].
HALIASSOS, A ;
CHOMEL, JC ;
TESSON, L ;
BAUDIS, M ;
KRUH, J ;
KAPLAN, JC ;
KITZIS, A .
NUCLEIC ACIDS RESEARCH, 1989, 17 (09) :3606-3606
[9]   Association between natural scrapie and PrP genotype in a flock of Suffolk sheep in Scotland [J].
Hunter, N ;
Moore, L ;
Hosie, BD ;
Dingwall, WS ;
Greig, A .
VETERINARY RECORD, 1997, 140 (03) :59-63
[10]   Natural scrapie and PrP genotype: case-control studies in British sheep [J].
Hunter, N ;
Goldmann, W ;
Foster, JD ;
Cairns, D ;
Smith, G .
VETERINARY RECORD, 1997, 141 (06) :137-140