Clinical experience with flow cytometric separation of human X- and Y-chromosome bearing sperm

被引：47

作者：

Fugger, EF ^{[1
]}

机构：

[1] Genet & IVF Inst, Fairfax, VA 22039 USA

[2] Virginia Commonwealth Univ, Med Coll Virginia, Richmond, VA 23298 USA

来源：

THERIOGENOLOGY | 1999年 / 52卷 / 08期

关键词：

flow cytometric separation; fluorescence in situ hybridization; human sperm; MicroSort;

D O I：

10.1016/S0093-691X(99)00228-9

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Numerous methods to separate human X- and Y-bearing sperm have been reported with unconfirmed separation after DNA analysis and inconsistent birth results. Successful flow cytometric separation of sperm resulting in alteration of the sex ratio of young born has been demonstrated in several animal species. Flow cytometric separation of human X- and Y-bearing sperm (MicroSort(R))(a) has been confirmed after DNA analysis by fluorescence in situ hybridization (FISH). Pregnancies and births have resulted from the use of MicroSort after intrauterine insemination (IUI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). (C) 1999 by Elsevier Science Inc.

引用

页码：1435 / 1440

页数：6

共 13 条

[1] Efficiency of sex pre-selection of spermatozoa by albumin separation method evaluated by double-labelled fluorescence in-situ hybridization [J].