Mechanisms of ammonia and ammonium transport by rhesus-associated glycoproteins

被引:43
作者
Caner, Tolga
Abdulnour-Nakhoul, Solange
Brown, Karen
Islam, M. Toriqul
Hamm, L. Lee
Nakhoul, Nazih L.
机构
[1] Southeast Louisiana Vet Hlth Care Syst, Dept Med, Nephrol Sect, New Orleans, LA USA
[2] Southeast Louisiana Vet Hlth Care Syst, Dept Physiol, New Orleans, LA USA
[3] Tulane Univ, Sch Med, Tulane Hypertens & Renal Ctr Excellence, New Orleans, LA 70112 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2015年 / 309卷 / 11期
基金
美国国家卫生研究院;
关键词
ammonia transport; Rh glycoproteins; pH regulation; RhAG; Rhbg; Rhcg; RH B GLYCOPROTEIN; INTRACELLULAR PH; HUMAN KIDNEY; RENAL RHBG; EXPRESSION; PROTEINS; CELLS; NH3; AQUAPORIN-1; CHANNEL;
D O I
10.1152/ajpcell.00085.2015
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In this study we characterized ammonia and ammonium (NH3/NH4+) transport by the rhesus- associated (Rh) glycoproteins RhAG, Rhbg, and Rhcg expressed in Xenopus oocytes. We used ionselective microelectrodes and two- electrode voltage clamp to measure changes in intracellular pH, surface pH, and whole cell currents induced by NH3/NH4+ and methyl amine/ammonium (MA/MA(+)). These measurements allowed us to define signal-specific signatures to distinguish NH3 from NH4+ transport and to determine how transport of NH3 and NH4+ differs among RhAG, Rhbg, and Rhcg. Our data indicate that expression of Rh glycoproteins in oocytes generally enhanced NH3/NH4+ transport and that cellular changes induced by transport of MA/MA(+) by Rh proteins were different from those induced by transport of NH3/NH4+. Our results support the following conclusions: 1) RhAG and Rhbg transport both the ionic NH4+ and neutral NH3 species; 2) transport of NH4+ is electrogenic; 3) like Rhbg, RhAG transport of NH4+ masks NH3 transport; and 4) Rhcg is likely to be a predominantly NH3 transporter, with no evidence of enhanced NH4+ transport by this transporter. The dual role of Rh proteins as NH3 and NH4+ transporters is a unique property and may be critical in understanding how transepithelial secretion of NH3/NH4+ occurs in the renal collecting duct.
引用
收藏
页码:C747 / C758
页数:12
相关论文
共 32 条
  • [1] NEUTRAL CARRIER BASED HYDROGEN-ION SELECTIVE MICROELECTRODE FOR EXTRACELLULAR AND INTRACELLULAR STUDIES
    AMMANN, D
    LANTER, F
    STEINER, RA
    SCHULTHESS, P
    SHIJO, Y
    SIMON, W
    [J]. ANALYTICAL CHEMISTRY, 1981, 53 (14) : 2267 - 2269
  • [2] Avent Neil D., 2006, Expert Reviews in Molecular Medicine, V8, P1, DOI 10.1017/S1462399406010969
  • [3] NH3 is involved in the NH4+ transport induced by the functional expression of the human RhC glycoprotein
    Bakouh, N
    Benjelloun, F
    Hulin, P
    Brouillard, F
    Edelman, A
    Chérif-Zahar, B
    Planelles, G
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (16) : 15975 - 15983
  • [4] Expression of the human erythroid Rh glycoprotein (RhAG) enhances both NH3 and NH4+ transport in HeLa cells
    Benjelloun, F
    Bakouh, N
    Fritsch, J
    Hulin, P
    Lipecka, J
    Edelman, A
    Planelles, G
    Thomas, SR
    Chérif-Zahar, B
    [J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2005, 450 (03): : 155 - 167
  • [5] INTRACELLULAR PH TRANSIENTS IN SQUID GIANT-AXONS CAUSED BY CO2, NH3, AND METABOLIC-INHIBITORS
    BORON, WF
    DEWEER, P
    [J]. JOURNAL OF GENERAL PHYSIOLOGY, 1976, 67 (01) : 91 - 112
  • [6] Expression of the ammonia transporter, RhC glycoprotein, in normal and neoplastic human kidney
    Han, Ki-Hwan
    Croker, Byron P.
    Clapp, William L.
    Werner, Dietrich
    Sahni, Manisha
    Kim, Jin
    Kim, Hye-Young
    Handlogten, Mary E.
    Weiner, I. David
    [J]. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2006, 17 (10): : 2670 - 2679
  • [7] Expression of the ammonia transporter family member, Rh B Glycoprotein, in the human kidney
    Han, Ki-Hwan
    Lee, Hyun-Wook
    Handlogten, Mary E.
    Whitehill, Florence
    Osis, Gunars
    Croker, Byron P.
    Clapp, William L.
    Verlander, Jill W.
    Weiner, I. David
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2013, 304 (07) : F972 - F981
  • [8] Expression of the gas-transporting proteins, Rh B glycoprotein and Rh C glycoprotein, in the murine lung
    Han, Ki-Hwan
    Mekala, Kavya
    Babida, Venetia
    Kim, Hye-Young
    Handlogten, Mary E.
    Verlander, Jill W.
    Weiner, I. David
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2009, 297 (01) : L153 - L163
  • [9] Expression of the ammonia transporter proteins RhB glycoprotein and RhC glycoprotein in the intestinal tract
    Handlogten, ME
    Hong, SP
    Zhang, L
    Vander, AW
    Steinbaum, ML
    Campbell-Thompson, M
    Weiner, ID
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2005, 288 (05): : G1036 - G1047
  • [10] The Rh protein family: gene evolution, membrane biology, and disease association
    Huang, Cheng-Han
    Ye, Mao
    [J]. CELLULAR AND MOLECULAR LIFE SCIENCES, 2010, 67 (08) : 1203 - 1218