Changes in gene expression profiles of human fibroblasts in response to sodium arsenite treatment

被引:69
作者
Yih, LH
Peck, K
Lee, TC [1 ]
机构
[1] Acad Sinica, Inst Biomed Sci, Taipei 115, Taiwan
[2] Natl Yang Ming Univ, Sch Life Sci, Inst Pharmacol, Taipei 112, Taiwan
关键词
D O I
10.1093/carcin/23.5.867
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Arsenic compounds are widely distributed and arsenic ingestion is associated with many human diseases, including blackfoot disease, atherosclerosis, and cancers. However, the underlying mechanism of arsenic toxicity is not understood. In human fibroblast cells (HFW), arsenite is known to induce oxidative damage, chromosome aberrations, cell cycle arrest, and aneuploidy, and the manifestation of these cellular responses is dependent on changes in gene expression which can be analyzed using the cDNA microarray technique. In this study, cDNA microarray membranes with 568 human genes were used to examine mRNA profile changes in HFW cells treated for 0 to 24 h with 5 muM sodium arsenite. On the basis of the mean value for three independent experiments, 133 target genes were selected for a 2 x 3 self-organizing map cluster analysis; 94 were found to be induced by arsenite treatment, whereas 39 were repressed. These genes were categorized as signal transduction, transcriptional regulation, cell cycle control, stress responses, proteolytic enzymes, and miscellaneous. Significant changes in the signaling-related and transcriptional regulation genes indicated that arsenite induces complex toxicopathological injury.
引用
收藏
页码:867 / 876
页数:10
相关论文
共 119 条
[21]   Deubiquitinating enzymes: Their diversity and emerging roles [J].
Chung, CH ;
Baek, SH .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 266 (03) :633-640
[22]  
Dang CV, 1999, MOL CELL BIOL, V19, P1
[23]   REACTIVE OXYGEN INTERMEDIATES TARGET CC(A/T)6GG SEQUENCES TO MEDIATE ACTIVATION OF THE EARLY GROWTH RESPONSE-1 TRANSCRIPTION FACTOR GENE BY IONIZING-RADIATION [J].
DATTA, R ;
TANEJA, N ;
SUKHATME, VP ;
QURESHI, SA ;
WEICHSELBAUM, R ;
KUFE, DW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (06) :2419-2422
[24]   IONIZING-RADIATION ACTIVATES TRANSCRIPTION OF THE EGR1 GENE VIA CARG ELEMENTS [J].
DATTA, R ;
RUBIN, E ;
SUKHATME, V ;
QURESHI, S ;
HALLAHAN, D ;
WEICHSELBAUM, RR ;
KUFE, DW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10149-10153
[25]   Arsenite inhibits Ras-dependent activation of ERK but activates ERK in the presence of oncogenic Ras in baboon vascular smooth muscle cells [J].
Daum, G ;
Pham, J ;
Deou, J .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2001, 217 (1-2) :131-136
[26]  
Davis SR, 2002, J NUTR, V132, P1085
[27]   Mammalian SWI-SNF complexes contribute to activation of the hsp70 gene [J].
De la Serna, IL ;
Carlson, KA ;
Hill, DA ;
Guidi, CJ ;
Stephenson, RO ;
Sif, S ;
Kingston, RE ;
Imbalzano, AN .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (08) :2839-2851
[28]   JUNB DIFFERS FROM C-JUN IN ITS DNA-BINDING AND DIMERIZATION DOMAINS, AND REPRESSES C-JUN BY FORMATION OF INACTIVE HETERODIMERS [J].
DENG, TL ;
KARIN, M .
GENES & DEVELOPMENT, 1993, 7 (03) :479-490
[29]   Arsenite blocks growth factor induced activation of the MAP kinase cascade, upstream of Ras and downstream of Grb2-Sos [J].
Doza, YN ;
Hall-Jackson, CA ;
Cohen, P .
ONCOGENE, 1998, 17 (01) :19-24
[30]   REDUCTION OF BACKGROUND PROBLEMS IN NONRADIOACTIVE NORTHERN AND SOUTHERN BLOT ANALYSES ENABLES HIGHER SENSITIVITY THAN P-32 BASED HYBRIDIZATIONS [J].
ENGLERBLUM, G ;
MEIER, M ;
FRANK, J ;
MULLER, GA .
ANALYTICAL BIOCHEMISTRY, 1993, 210 (02) :235-244