Yellow leaf of sugarcane is caused by at least three different genotypes of sugarcane yellow leaf virus, one of which predominates on the Island of Reunion

被引:47
作者
Ahmad, Y. Abu
Rassaby, L.
Royer, M.
Borg, Z.
Braithwaite, K. S.
Mirkov, T. E.
Irey, M. S.
Perrier, X.
Smith, G. R.
Rott, P.
机构
[1] CIRAD, UMR 385, BGPI, INRA,Ctr Cooperat Int Rech Agron Dev, F-34398 Montpellier 5, France
[2] BSES Ltd, David N Plant Res Ctr, Indooroopilly, Qld, Australia
[3] Texas A&M Univ, Agr Expt Stn, Dept Plant Pathol & Microbiol, Weslaco, TX USA
[4] US Sugar Corp, Clewiston, FL USA
[5] Cooperat Int Rech Agron Dev, Multiplicat Vegetat Ctr, UPR 75, Montpellier, France
关键词
D O I
10.1007/s00705-005-0712-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genetic diversity of sugarcane yellow leaf virus (SCYLV) was analyzed with 43 virus isolates from Reunion Island and 17 isolates from world-wide locations. We attempted to amplify by reverse-transcription polymerase chain reaction (RT-PCR), clone, and sequence four different fragments covering 72% of the genome of these virus isolates. The number of amplified isolates and useful sequence information varied according to each fragment, whereas an amplicon was obtained with diagnostic primers for 59 out of 60 isolates (98%). Phylogenetic analyses of the sequences determined here and additional sequences of 11 other SCYLV isolates available from GenBank showed that SCYLV isolates were distributed in different phylogenetic groups or belonged to single genotypes. The majority of isolates from Reunion Island were grouped in phylogenetic clusters that did not contain any isolates from other origins. The complete six ORFs (5612 bp) of five SCYLV isolates (two from Reunion Island, one from Brazil, one from China, and one from Peru) were amplified, cloned, and sequenced. The existence of at least three distinct genotypes of SCYLV was shown by phylogenetic analysis of the sequences of these isolates and additional published sequences of three SCYLV isolates (GenBank accessions). The biological significance of these genotypes and of the origin of the distinct lineage of SCYLV in Reunion Island remains to be determined.
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页码:1355 / 1371
页数:17
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