Chimerism analysis after allogeneic bone marrow transplantation with nonradioactive RFLP and PCR-AFLP using the same DNA

被引：4

作者：

Schreiner, T

ProchnowCalzia, H

Maccari, B

Erne, E

Kinzler, I

Wolpl, A

Wiesneth, M

机构：

[1] UNIV ULM,RED CROSS BLOOD CTR,ULM,GERMANY

[2] UNIV ULM,DEPT TRANSFUS MED,D-7900 ULM,GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1996年 / 196卷 / 01期

关键词：

chimerism analysis; bone marrow transplantation; restriction fragment length polymorphism analysis; polymerase chain reaction amplified fragment length polymorphism analysis;

D O I：

10.1016/0022-1759(96)00120-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method combining RFLP and PCR-AFLP analyses for studying chimerism after allogeneic bone marrow transplantation. Using RFLP analysis alone with DNA probe hMF1 87% of 244 donor/recipient pairs revealed different specific bands. By examination of the identical DNA probes using PCR-AFLP, an additional 52% of the residual donor/recipient pairs could be identified. Thus, 94% of allogeneic transplantations can be monitored using a combination of RFLP and PCR-AFLP analyses at one locus.

引用

页码：93 / 96

页数：4

共 10 条

[1] A RAPID PROCEDURE FOR EXTRACTING GENOMIC DNA FROM LEUKOCYTES [J].