A rapid and sensitive fluorescence biosensor for Hg2+ detection in environmental samples

被引：15

作者：

Xing, Yunpeng ^{[1
,2
]}

Xue, Boyuan ^{[2
]}

Qi, Peishi ^{[1
]}

Chen, George Y. ^{[3
]}

Zhou, Xiaohong ^{[2
]}

机构：

[1] Harbin Inst Technol, Sch Environm, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China

[2] Tsinghua Univ, Sch Environm, State Key Joint Lab ESPC, Beijing 100084, Peoples R China

[3] Shenzhen Univ, Guangdong & Hong Kong Joint Res Ctr Opt Fibre Sen, Shenzhen Key Lab Photon Devices & Sensing Syst In, Shenzhen 518060, Peoples R China

来源：

SENSORS AND ACTUATORS REPORTS | 2022年 / 4卷

关键词：

T-Hg 2 + -T mismatch; SYBR Green I; Mercury-specific oligonucleotide; Mercury detection; Fluorescence intensity; LABEL-FREE DETECTION; SELECTIVE DETECTION; GOLD NANOPARTICLES; DNA BIOSENSOR; ION DETECTION; MERCURY(II); SENSOR; HG(II); PROBE; LEAD;

D O I：

10.1016/j.snr.2022.100101

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

As a significant environmental pollutant, Hg2+ has gained widespread concern around the world. In our work, a rapid and elegant fluorescence method based on SYBR GREEN I (SGI) and mercury-specific oligonucleotide (MSO) has been developed for detection of Hg2+. Utilizing the T-Hg2+-T mismatch principle, MSO forms a double-stranded hairpin structure, which can readily embed SGI and produce strong fluorescence. Under optimized conditions including MSO concentration, dye/MSO base ratio, pH value, ionic strength, reaction time and incubation time, a wide linear range (10-100 nM) and a low limit of detection (0.68 nM) were demonstrated. Almost no statistically significant interference for Hg2+ detection was observed among the possible coexisting substances in the water samples, including 15 factors. The recoveries of the three environmental water samples were in the range of 82.8% to 101.8%, indicating that the method was only weakly affected by the environmental matrix and could be applied to the detection of Hg2+ in environmental water samples. This method features potentially low cost, rapid processing, and convenient operation, which indicates considerable promise in practical Hg2+ monitoring.

引用

页数：8

共 41 条

[1] A novel SPR based Fe@Ag core-shell nanosphere entrapped on starch matrix an optical probe for sensing of mercury(II) ion: A nanomolar detection, wide pH range and real water sample application [J].