nagZ Triggers Gonococcal Biofilm Disassembly

被引：26

作者：

Bhoopalan, Senthil V. ^{[1
]}

Piekarowicz, Andrzej ^{[1
]}

Lenz, Jonathan D. ^{[2
]}

Dillard, Joseph P. ^{[2
]}

Stein, Daniel C. ^{[1
]}

机构：

[1] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA

[2] Univ Wisconsin, Dept Med Microbiol & Immunol, Sch Med & Publ Hlth, Madison, WI 53706 USA

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

美国国家卫生研究院;

关键词：

BETA-N-ACETYLGLUCOSAMINIDASE; NEISSERIA-GONORRHOEAE; ESCHERICHIA-COLI; STAPHYLOCOCCUS-EPIDERMIDIS; SUBCELLULAR-LOCALIZATION; INTERCELLULAR ADHESIN; TRANSFORMATION; RESISTANCE; STRAIN; DNA;

D O I：

10.1038/srep22372

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bacterial-bacterial interactions play a critical role in promoting biofilm formation. Here we show that NagZ, a protein associated with peptidoglycan recycling, has moonlighting activity that allows it to modulate biofilm accumulation by Neisseria gonorrhoeae. We characterize the biochemical properties of NagZ and demonstrate its ability to function as a dispersing agent for biofilms formed on abiotic surfaces. We extend these observations to cell culture and tissue explant models and show that in nagZ mutants, the biofilms formed in cell culture and on human tissues contain significantly more biomass than those formed by a wild-type strain. Our results demonstrate that an enzyme thought to be restricted to peptidoglycan recycling is able to disperse preformed biofilms.

引用

页数：10

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