Kinjiso (Gynura bicolor DC.) colored extract induce apoptosis in HL60 leukemia cells

Kinjiso (Gynura bicolor DC.) is a traditional vegetable in Ishikawa prefecture. Kinjiso hot water extract containing the natural colored compounds anthocyanins was fractionated into the adsorbed-and the non-adsorbed fractions by SepPakC18 column chromatography. Kinjiso hot water extract and the SepPakC18 column adsorbed fraction showed the inhibitory effect of the HL60 human leukemia cell growth and the scavenging activity towards DPPH radical. Both effects of the SepPak C18 column adsorbed fraction were higher than those of the hot water extract. The anthocyans peralgonidin, delphinidin, malvidin and oenin (malvidin 3-glucoside) inhibited the HL60 cell growth. The Kinjiso SepPakC18 column adsorbed fraction and the anthocyanidins peralgonidin, delphinidin and malvidin induced the apoptotic cell bodies and oligonucleosomal DNA fragmentation in HL60 cells. The results indicated that the Kinjiso SepPakC18 column adsorbed fraction and the anthocyanidins induced apoptosis in HL60 cells. Kinjiso SepPakC18 column adsorbed fraction was further fractionated by Sephadex LH20 column chromatography. Both of the fractions that containing large and small amount of anthocyanins inhibited cell growth and induced apoptosis in HL60 cells. The result suggests that Kinjiso SepPakC18 column adsorbed fraction contain not only anthocyanins but also other components induced the apoptosis in HL60 cells. The SepPakC18 column adsorbed fraction is possibly used as the coloring additive that shows the radical scavenging activity and apoptosis inducing effect in cancer cells.