The aim of this study was to examine the response of cell-mediated ovarian immunity against Salmonella infection in hens. Laying hens were injected intraperitoneally with PBS (control) or Salmonella enteritidis (SE). Ovarian stroma containing stromal follicles, small white follicles (SWF), and third largest (F3) and the largest (F1) follicles were collected 12 or 24 h after inoculation and fixed in periodate-lysine-paraformaldehyde. Frozen sections were stained first for CD3+, CD4+, or CD8+ T cells and then for SE by a double immunostaining method. Immunoreaction products for SE were detected in the ovarian stroma, theca of stromal follicles, SWF, F3, and F1 at 12 and 24 h after inoculation. Immunopositive T-cell subsets were localized in the stroma and theca of follicles in birds inoculated with or without SE. The populations of CD3+, CD4+, and CD8+ T cells were significantly greater in the stroma and the theca of follicles 12 h after SE inoculation than in those of control birds (P < 0.01). Their frequencies were further increased in those tissues 24 h after inoculation (P < 0.01). Injection of SE did not cause significant differences in the CD4+:CD8+ T-cell ratio as both subsets increased proportionately. The current results indicate that the population of T-cell subsets increases in the ovarian stroma and the follicular tissues in response to SE invasion within 12 h of inoculation. Thus, cell-mediated immune response against SE, their products, or both may be induced in the hen ovary.
