The C. elegans lifespan assay toolkit

被引：92
作者：
Amrit, Francis Raj Gandhi [1 ]
Ratnappan, Ramesh [1 ]
Keith, Scott Alexander [1 ]
Ghazi, Arjumand [1 ]
机构：
[1] Univ Pittsburgh, Childrens Hosp Pittsburgh, Rangos Res Ctr 7129, Sch Med,Dept Pediat, Pittsburgh, PA 15224 USA
来源：
METHODS | 2014年 / 68卷 / 03期
关键词：
Aging; Lifespan; C; elegans; Stress assay; High throughput; Longevity; CAENORHABDITIS-ELEGANS; GENETIC INTERFERENCE; RNA INTERFERENCE; BACTERIAL; LONGEVITY; PATHWAYS; MUTANT; RESTRICTION; POPULATIONS; INFECTION;
D O I：
10.1016/j.ymeth.2014.04.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Since the discovery of single gene mutations that double its lifespan, the nematode Caenorhabditis elegans has provided remarkable insights into the biology of aging. The precisely measurable lifespan of worms has proven to be an efficient tool to assess the impact of various genetic, physiological and environmental factors on organismal aging. In this article, we describe methods to set up and monitor experiments to determine worm lifespan. We include procedures used for classical, small-scale lifespan assays that are generally performed on solid media, and review recent advances in high-throughput, automated longevity experiments conducted in liquid culture and microfiuidic devices. In addition, tools that help analyze this data to obtain survival statistics are summarized, and C elegans strains that offer particular advantages for lifespan studies are listed. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：465 / 475
页数：11
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