Cell-Instructive Microgels with Tailor-Made Physicochemical Properties

被引：54

作者：

Allazetta, Simone ^{[1
]}

Kolb, Laura ^{[1
]}

Zerbib, Samantha ^{[1
]}

Bardy, Jo'an ^{[1
]}

Lutolf, Matthias P. ^{[1
,2
]}

机构：

[1] Ecole Polytech Fed Lausanne, Sch Life Sci & Sch Engn, Inst Bioengn, Lab Stem Cell Bioengn, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Sch Basic Sci, Inst Chem Sci & Engn, CH-1015 Lausanne, Switzerland

来源：

SMALL | 2015年 / 11卷 / 42期

基金：

瑞士国家科学基金会;

关键词：

PLURIPOTENT STEM-CELLS; MICHAEL-TYPE ADDITION; DROPLET MICROFLUIDICS; HYDROGELS; 3D; TISSUE; CULTURE; ENCAPSULATION; MIGRATION; CANCER;

D O I：

10.1002/smll.201501001

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A microfluidic in vitro cell encapsulation platform to systematically test the effects of microenvironmental parameters on cell fate in 3D is developed. Multiple cell types including fibroblasts, embryonic stem cells, and cancer cells are incorporated in enzymatically cross-linked poly(ethylene glycol)-based microgels having defined and tunable mechanical and biochemical properties. Furthermore, different approaches to prevent cell "escape" from the microcapsules are explored and shown to substantially enhance the potential of this technology. Finally, coencapsulation of microgels within nondegradable gels allows cell viability, proliferation, and morphology to be studied in different microenvironmental conditions up to two weeks in culture.

引用

页码：5647 / 5656

页数：10

共 32 条

[31] Modeling tissue morphogenesis and cancer in 3D [J].