Cell-Instructive Microgels with Tailor-Made Physicochemical Properties

被引:54
作者
Allazetta, Simone [1 ]
Kolb, Laura [1 ]
Zerbib, Samantha [1 ]
Bardy, Jo'an [1 ]
Lutolf, Matthias P. [1 ,2 ]
机构
[1] Ecole Polytech Fed Lausanne, Sch Life Sci & Sch Engn, Inst Bioengn, Lab Stem Cell Bioengn, CH-1015 Lausanne, Switzerland
[2] Ecole Polytech Fed Lausanne, Sch Basic Sci, Inst Chem Sci & Engn, CH-1015 Lausanne, Switzerland
基金
瑞士国家科学基金会;
关键词
PLURIPOTENT STEM-CELLS; MICHAEL-TYPE ADDITION; DROPLET MICROFLUIDICS; HYDROGELS; 3D; TISSUE; CULTURE; ENCAPSULATION; MIGRATION; CANCER;
D O I
10.1002/smll.201501001
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A microfluidic in vitro cell encapsulation platform to systematically test the effects of microenvironmental parameters on cell fate in 3D is developed. Multiple cell types including fibroblasts, embryonic stem cells, and cancer cells are incorporated in enzymatically cross-linked poly(ethylene glycol)-based microgels having defined and tunable mechanical and biochemical properties. Furthermore, different approaches to prevent cell "escape" from the microcapsules are explored and shown to substantially enhance the potential of this technology. Finally, coencapsulation of microgels within nondegradable gels allows cell viability, proliferation, and morphology to be studied in different microenvironmental conditions up to two weeks in culture.
引用
收藏
页码:5647 / 5656
页数:10
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