miRNA Expression in CHO: Nature knows best

被引：6

作者：

Baik, Jong Youn ^{[1
,2
]}

Lee, Kelvin H. ^{[1
,2
]}

机构：

[1] Univ Delaware, Dept Chem & Biomol Engn, Newark, DE 19716 USA

[2] Univ Delaware, Delaware Biotechnol Inst, Newark, DE USA

来源：

BIOTECHNOLOGY JOURNAL | 2014年 / 9卷 / 04期

基金：

奥地利科学基金会;

关键词：

HAMSTER OVARY CELLS; GENE;

D O I：

10.1002/biot.201300503

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Improvement of Chinese hamster ovary (CHO) cells for therapeutic protein production has great potential for the manufacturing of biopharmaceuticals. This commentary by Baik and Lee discusses the study by Klanert et al., which describes an improved tool that will allow greater control over the design of CHO cells. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：459 / 460

页数：2

共 8 条

[1] Proteomic Analysis of Chinese Hamster Ovary Cells [J].

Baycin-Hizal, Deniz ;

Tabb, David L. ;

Chaerkady, Raghothama ;

Chen, Lily ;

Lewis, Nathan E. ;

Nagarajan, Harish ;

Sarkaria, Vishaldeep ;

Kumar, Amit ;

Wolozny, Daniel ;

Colao, Joe ;

Jacobson, Elena ;

Tian, Yuan ;

O'Meally, Robert N. ;

Krag, Sharon S. ;

Cole, Robert N. ;

Palsson, Bernhard O. ;

Zhang, Hui ;

Betenbaugh, Michael .

JOURNAL OF PROTEOME RESEARCH, 2012, 11 (11) :5265-5276

[2] Transcriptional silencing is associated with extensive methylation of the CMV promoter following adenoviral gene delivery to muscle [J].

Brooks, AR ;

Harkins, RN ;

Wang, PY ;

Qian, HS ;

Liu, PX ;

Rubanyi, GM .

JOURNAL OF GENE MEDICINE, 2004, 6 (04) :395-404

[3] High-level expression of proteins in mammalian cells using transcription regulatory sequences from the Chinese hamster EF-1α gene [J].

Deer, JR ;

Allison, DS .

BIOTECHNOLOGY PROGRESS, 2004, 20 (03) :880-889

[4] CHO microRNA engineering is growing up: Recent successes and future challenges [J].

Jadhav, Vaibhav ;

Hackl, Matthias ;

Druz, Aliaksandr ;

Shridhar, Smriti ;

Chung, Cheng-Yu ;

Heffner, Kelley M. ;

Kreil, David P. ;

Betenbaugh, Mike ;

Shiloach, Joseph ;

Barron, Niall ;

Grillari, Johannes ;

Borth, Nicole .

BIOTECHNOLOGY ADVANCES, 2013, 31 (08) :1501-1513

[5] A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells [J].

Jadhav, Vaibhav ;

Hackl, Matthias ;

Bort, Juan A. Hernandez ;

Wieser, Matthias ;

Harreither, Eva ;

Kunert, Renate ;

Borth, Nicole ;

Grillari, Johannes .

BIOTECHNOLOGY AND BIOENGINEERING, 2012, 109 (06) :1376-1385

[6] Endogenous microRNA clusters outperform chimeric sequence clusters in Chinese hamster ovary cells [J].

Klanert, Gerald ;

Jadhav, Vaibhav ;

Chanoumidou, Konstantina ;

Grillari, Johannes ;

Borth, Nicole ;

Hackl, Matthias .

BIOTECHNOLOGY JOURNAL, 2014, 9 (04) :538-544

[7] Genomics in mammalian cell culture bioprocessing [J].

Wuest, Diane M. ;

Harcum, Sarah W. ;

Lee, Kelvin H. .

BIOTECHNOLOGY ADVANCES, 2012, 30 (03) :629-638

[8] The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line [J].

Xu, Xun ;

Nagarajan, Harish ;

Lewis, Nathan E. ;

Pan, Shengkai ;

Cai, Zhiming ;

Liu, Xin ;

Chen, Wenbin ;

Xie, Min ;

Wang, Wenliang ;

Hammond, Stephanie ;

Andersen, Mikael R. ;

Neff, Norma ;

Passarelli, Benedetto ;

Koh, Winston ;

Fan, H. Christina ;

Wang, Jianbin ;

Gui, Yaoting ;

Lee, Kelvin H. ;

Betenbaugh, Michael J. ;

Quake, Stephen R. ;

Famili, Iman ;

Palsson, Bernhard O. ;

Wang, Jun .

NATURE BIOTECHNOLOGY, 2011, 29 (08) :735-U131

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