Genetic diversity and phylogenetic relationships among and within populations of Whitmania pigra and Hirudo nipponica based on ISSR and SRAP markers

被引：12

作者：

Liu, Fei ^{[1
,2
,3
]}

Guo, Qiao-Sheng ^{[1
]}

Shi, Hong-Zhuan ^{[1
]}

Wang, Tian ^{[3
]}

Zhu, Zai-Biao ^{[1
]}

机构：

[1] Nanjing Agr Univ, Inst Chinese Med Mat, Nanjing 210095, PR, Peoples R China

[2] Yancheng Inst Technol, Sch Chem & Biol Engn, Yancheng 224051, Peoples R China

[3] Nanjing Agr Univ, Dept Anim Nutr & Feed Sci, Coll Anim Sci & Technol, Nanjing 210095, Peoples R China

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2013年 / 51卷

关键词：

Whitmania pigra; Hirudo nipponica; Molecular marker; Phylogenetic relationships; Genetic diversity; FINGERPRINTING TECHNIQUES RAPD; MICROSATELLITE MARKERS; POLYMORPHISM; AFLP; SYSTEM; LEECH; FLOW; SSR; L; DISTANCE;

D O I：

10.1016/j.bse.2013.08.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The objective of this study was to obtain an overview of the genetic relationships among and within Whitmania pigra and Hirudo nipponica using the ISSR and SRAP markers that were derived from related accessions, 11 W pigra and 4 H. nipponica, from 8 provinces in China. All populations were uniquely fingerprinted by two markers. Mean genetic similarities were estimated at 0.76 and 0.79 using the ISSRs and SRAPs. Two main clusters that separated the W pigra and H. nipponica populations were produced using UPGMA analysis. The Mantel test resulted in a good fit (SRAP = 0.80609) to the fit (ISSR = 0.76006) of the cophenetic values. Comparing the two marker systems, the ISSR and SRAP similarity indices were correlated (r = 0.8564). Finally, an appropriate strategy for conserving the Hirudo germplasm is proposed. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：215 / 223

页数：9

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