Towards efficient crude oil degradation by a mixed bacterial consortium

被引：336

作者：

Rahman, KSM

Thahira-Rahman, J

Lakshmanaperumalsamy, P

Banat, IM

机构：

[1] Univ Ulster, Sch Biol & Environm Studies, Biotechnol Res Grp, Coleraine BT52 1SA, Londonderry, North Ireland

[2] Bharathiar Univ, Dept Environm Sci, Coimbatore 641046, Tamil Nadu, India

来源：

BIORESOURCE TECHNOLOGY | 2002年 / 85卷 / 03期

关键词：

biodegradation; crude oil; mixed bacterial consortium; optimum conditions;

D O I：

10.1016/S0960-8524(02)00119-0

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A laboratory study was undertaken to assess the optimal conditions for biodegradation of Bombay High (BH) crude oil. Among 130 oil degrading bacterial cultures isolated from oil contaminated soil samples, Micrococcus sp. GS2-22, Corynebacterium sp. GS5-66, Flavobacterium sp. DS5-73, Bacillus sp. DS6-86 and Pseudomonas sp. DS10-129 were selected for the study based on the efficiency of crude oil utilisation. A mixed bacterial consortium prepared using the above strains was also used. Individual bacterial cultures showed less growth and degradation than did the mixed bacterial consortium. At 1% crude oil concentration, the mixed bacterial consortium degraded a maximum of 78% of BH crude oil. This was followed by 66% by Pseudomonas sp. DS10-129, 59% by Bacillus sp. DS6-86, 49% by Micrococcus sp. GS2-22, 43% by Corynebacterium sp. GS5-66 and 41% by Flavobacterium sp. DS5-73. The percentage of degradation by the mixed bacterial consortium decreased from 78% to 52% as the concentration of crude oil was increased from 1% to 10%. Temperature of 30 degreesC and pH 7.5 were found to be optima for maximum biodegradation. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：257 / 261

页数：5

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