Single-molecule Fluorescence Imaging of Kinesin Using Linear Zero-Mode Waveguides

被引：0

作者：

Morita, Yuki ^{[1
]}

Fujimoto, Kazuya ^{[1
]}

Iino, Ryota ^{[2
]}

Tomishige, Michio ^{[3
]}

Shintaku, Hirofumi ^{[1
]}

Kotera, Hidetoshi ^{[1
]}

Yokokawa, Ryuji ^{[1
]}

机构：

[1] Kyoto Univ, Dept Micro Engn, Kyoto, Japan

[2] Inst Mol Sci, Okazaki, Aichi, Japan

[3] Univ Tokyo, Dept Engn Phys, Tokyo, Japan

来源：

2016 IEEE SENSORS | 2016年

关键词：

Kinesin; Single-molecule analysis; Linear Zero-Mode Waveguides; Microtubule; MYOSIN;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

For the analysis of kinesin motility, we demonstrate an observation system to simultaneously visualize kinesin and ATP molecules at the single molecule level using Linear Zero-Mode Waveguides (LZMWs). LZMWs allow fluorescent imaging at up to micromolar concentration by confining the excitation volume, which is high enough for studying enzymatic reactions such as ATP hydrolysis. LZMWs were fabricated by electron-beam lithography and lift-off process. Moreover, we developed a method to immobilize microtubules in LZMWs to observe kinesin motility.

引用

页数：3

共 4 条

[1] The kinetic mechanism of kinesin [J].