Salivary flow rate and biochemical composition analysis in stimulated whole saliva of children with cystic fibrosis

Objective: It is recognized that cystic fibrosis (CF) patients present a risk for oral diseases, since it affects exocrine glands, and the treatment consists of a carbohydrate-rich diet. Recognizing the protective function of saliva on maintaining oral health, the aim of the study was to evaluate salivary parameters in stimulated whole saliva from children with CF. Methods: A case-control study was conducted comparing stimulated whole saliva of healthy (n = 28; control group) and CF children (n = 21; experimental group). Salivary flow rate, initial pH, buffer capacity (total and in each range of pH), total protein and sialic acid (total, free, and conjugated) concentration, a-amylase and salivary peroxidase activities were evaluated. Data were compared by two-tailed Student t test (95% CI; p <= 0.05). Results: CF patients presented a significant reduction in salivary parameters compared with the control group (p <= 0.05): salivary flow rate (36%), buffer capacity (pH range from 6.9 to 6.0), sialic acid concentration (total 75%, free 61%, and conjugated 83%); alpha-amylase and salivary peroxidase activities (55%). Additionally, a significant increase in total protein concentration (180%) of stimulated whole saliva from CF patients was verified compared with the control group (p <= 0.05). Conclusion: Children with CF presented significant changes in salivary composition, including salivary flow rate, buffering capacity and protective proteins of the oral cavity, compared with children without (C). 2015 Elsevier Ltd. All rights reserved.