Behavior-associated Neuronal Activation After Kainic Acid-induced Hippocampal Neurotoxicity is Modulated in Time

被引:6
作者
Aguilar-Arredondo, Andrea [1 ]
Lopez-Hernandez, Fernanda [1 ]
Garcia-Velazquez, Lizbeth [1 ]
Arias, Clorinda [1 ]
Zepeda, Angelica [1 ]
机构
[1] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Med Genom & Toxicol Ambiental, AP 70-228, Mexico City 04510, DF, Mexico
来源
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2017年 / 300卷 / 02期
关键词
hippocampal plasticity; dentate gyrus; synaptophysin; immediate early gene; spatial-exploratory behavior; injury; intrahippocampal kainic acid; TEMPORAL-LOBE EPILEPSY; IMMEDIATE-EARLY GENE; ARC MESSENGER-RNA; DENTATE GYRUS; SPATIAL EXPLORATION; INTRAHIPPOCAMPAL INJECTION; PROTEIN EXPRESSION; ALZHEIMERS-DISEASE; STATUS EPILEPTICUS; GRANULE CELLS;
D O I
10.1002/ar.23513
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Kainic acid-induced (KA) hippocampal damage leads to neuronal death and further synaptic plasticity. Formation of aberrant as well as of functional connections after such procedure has been documented. However, the impact of such structural plasticity on cell activation along time after damage and in face of a behavioral demand has not been explored. We evaluated if the mRNA and protein levels of plasticity-related protein synaptophysin (Syp and SYP, respectively) and activity-regulated cytoskeleton-associated protein mRNA and protein levels (Arc and Arc, respectively) in the dentate gyrus were differentially modulated in time in response to a spatial-exploratory task after KA-induced hippocampal damage. In addition, we analyzed Arc+/NeuN+ immunopositive cells in the different experimental conditions. We infused KA intrahippocampally to young-adult rats and 10 or 30 days post-lesion (dpl) animals performed a hippocampus-activating spatial-exploratory task. Our results show that Syp mRNA levels significantly increase at 10dpl and return to control levels after 30dpl, whereas SYP protein levels are diminished at 10dpl, but significantly increase at 30dpl, as compared to 10dpl. Arc mRNA and protein levels are both increased at 30dpl as compared to sham. Also the number of NeuN+/Arc+ cells significantly increases at 30dpl in the group with a spatial-exploratory demand. These results provide information on the long-term modifications associated to structural plasticity and neuronal activation in the dentate gyrus after excitotoxic damage and in face of a spatial-exploratory behavior. Anat Rec, 300:425-432, 2017. (c) 2016 Wiley Periodicals, Inc.
引用
收藏
页码:425 / 432
页数:8
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