Serum- and glucocorticoid-inducible kinases (SGK) regulate KCNQ1/KCNE potassium channels

被引:9
作者
Strutz-Seebohm, Nathalie [1 ,2 ]
Henrion, Ulrike [2 ]
Steinke, Katja [1 ]
Tapken, Daniel [1 ]
Lang, Florian [2 ]
Seebohm, Guiscard [1 ]
机构
[1] Ruhr Univ Bochum, Dept Biochem, D-44780 Bochum, Germany
[2] Univ Tubingen, Dept Physiol I, Tubingen, Germany
关键词
KCNQ1; xenopus; trafficking; modulation; kinase; voltage clamp; structure; model; biophysics; ion; SUBUNITS; REQUIREMENT; K(V)LQT1; PROTEINS; FORM;
D O I
10.4161/chan.3.2.8086
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The stress reaction includes the release of stress hormones such as cortisol via the HPA axis4. One of the genes regulated by cortisol is the serum- and glucocorticoid-inducible kinase 1 (SGK1) a stimulator of the slow outward potassium channel KCNQ1/KCNE1 - one of the major mediators of cardiac repolarization. Apart from KCNE1, several other KCNE beta subunits including KCNE3 and KCNE5 have been detected at the mRNA level in cardiac tissue as well as in the inner ear and the gastrointestinal tract. Here, we extend our previous investigations to KCNQ1/KCNE3 channels and their modulation by SGKs. We show that these channels are not stimulated by any of the three SGK isoforms when expressed in a heterologous expression system. 3D docking simulations suggest that crucial residues within KCNQ1 and KCNE1 are co-localized to a region close to the putative inner phase of the membrane, suggesting a key region important for channel complex sorting into vesicles. Identification of the KCNQ1/KCNE recycling pathway and its modulation by SGK provides a mechanistic insight into stress-induced modulation of KCNQ1/KCNE channels.
引用
收藏
页码:88 / 90
页数:3
相关论文
共 25 条
[1]   K(v)LQT1 and IsK (minK) proteins associate to form the I-Ks cardiac potassium current [J].
Barhanin, J ;
Lesage, F ;
Guillemare, E ;
Fink, M ;
Lazdunski, M ;
Romey, G .
NATURE, 1996, 384 (6604) :78-80
[2]   In vitro molecular interactions and distribution of KCNE family with KCNQ1 in the human heart [J].
Bendahhou, S ;
Marionneau, C ;
Haurogne, K ;
Larroque, MM ;
Derand, R ;
Szuts, V ;
Escande, D ;
Demolombe, S ;
Barhanin, J .
CARDIOVASCULAR RESEARCH, 2005, 67 (03) :529-538
[3]   Association of the serum and glucocorticoid regulated kinase (sgk1) gene with QT interval [J].
Busjahn, A ;
Seebohm, G ;
Maier, G ;
Toliat, MR ;
Nürnberg, P ;
Aydin, A ;
Luft, FC ;
Lang, F .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2004, 14 (03) :135-142
[4]   The N-terminal juxtamembranous domain of KCNQ1 is critical for channel surface expression -: Implications in the Romano-Ward LQT1 syndrome [J].
Dahimene, Shehrazade ;
Alcolea, Sebastien ;
Naud, Patrice ;
Jourdon, Philippe ;
Escande, Denis ;
Brasseur, Robert ;
Thomas, Annick ;
Baro, Isabelle ;
Merot, Jean .
CIRCULATION RESEARCH, 2006, 99 (10) :1076-1083
[5]   Colocalization of KCNQ1/KCNE channel subunits in the mouse gastrointestinal tract [J].
Dedek, K ;
Waldegger, S .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2001, 442 (06) :896-902
[6]   Differential expression of KvLQT1 and its regulator IsK in mouse epithelia [J].
Demolombe, S ;
Franco, D ;
De Boer, P ;
Kuperschmidt, S ;
Roden, D ;
Pereon, Y ;
Jarry, A ;
Moorman, AFM ;
Escande, D .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2001, 280 (02) :C359-C372
[7]  
ELIOT RS, 1995, J AM COLL CARDIOL, V5, P95
[8]   Regulation of KCNE1-dependent K+ current by the serum and glucocorticoid-inducible kinase (SGK) isoforms [J].
Embark, HM ;
Böhmer, C ;
Vallon, V ;
Luft, F ;
Lang, F .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2003, 445 (05) :601-606
[9]   The cardiac K+ channel KCNQ1 is essential for gastric acid secretion [J].
Grahammer, F ;
Herling, AW ;
Lang, HJ ;
Schmitt-Gräff, A ;
Wittekindt, OH ;
Nitschke, R ;
Bleich, M ;
Barhanin, J ;
Warth, R .
GASTROENTEROLOGY, 2001, 120 (06) :1363-1371
[10]   Basolateral localisation of KCNQ1 potassium channels in MDCK cells: molecular identification of an N-terminal targeting motif [J].
Jespersen, T ;
Rasmussen, HB ;
Grunnet, M ;
Jensen, HS ;
Angelo, K ;
Dupuis, DS ;
Vogel, LK ;
Jorgensen, NK ;
Klaerke, DA ;
Olesen, SP .
JOURNAL OF CELL SCIENCE, 2004, 117 (19) :4517-4526