Wide-field Raman imaging of dental lesions

被引:25
作者
Yang, Shan [1 ]
Li, Bolan [1 ]
Akkus, Anna [2 ]
Akkus, Ozan [1 ,3 ,4 ]
Lang, Lisa [2 ]
机构
[1] Case Western Reserve Univ, Dept Mech & Aerosp Engn, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Sch Dent Med, Cleveland, OH 44106 USA
[3] Case Western Reserve Univ, Sch Med, Dept Orthopaed, Cleveland, OH 44106 USA
[4] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA
关键词
ENAMEL; CARIES; REMINERALIZATION; BIOLOGY;
D O I
10.1039/c4an00164h
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Detection of dental caries at the onset remains as a great challenge in dentistry. Raman spectroscopy could be successfully applied towards detecting caries since it is sensitive to the amount of Raman active mineral crystals, the most abundant component of enamel. Effective diagnosis requires full examination of a tooth surface via Raman mapping. Point-scan Raman mapping is not clinically relevant (feasible) due to lengthy data acquisition time. In this work, a wide-field Raman imaging system was assembled based on a high-sensitivity 2D CCD camera for imaging the mineralization status of teeth with lesions. Wide-field images indicated some lesions to be hypomineralized and others to be hypermineralized. The observations of wide-field Raman imaging were in agreement with point-scan Raman mapping. Therefore, sound enamel and lesions can be discriminated by Raman imaging of the mineral content. In conclusion, wide-field Raman imaging is a potentially useful tool for visualization of dental lesions in the clinic.
引用
收藏
页码:3107 / 3114
页数:8
相关论文
共 21 条
[1]   Emerging technologies for diagnosis of dental caries: The road so far [J].
Amaechi, Bennett T. .
JOURNAL OF APPLIED PHYSICS, 2009, 105 (10)
[2]   INHIBITION OF ENAMEL DEMINERALIZATION BY ALBUMIN INVITRO [J].
ARENDS, J ;
SCHUTHOF, J ;
CHRISTOFFERSEN, J .
CARIES RESEARCH, 1986, 20 (04) :337-340
[3]   Ultrastructural changes accompanying the mechanical deformation of bone tissue: A Raman imaging study [J].
Carden, A ;
Rajachar, RM ;
Morris, MD ;
Kohn, DH .
CALCIFIED TISSUE INTERNATIONAL, 2003, 72 (02) :166-175
[4]  
Centers for Disease Control and Prevention, 2011, OR HLTH PREV CAV GUM
[5]   New Approaches to Enhanced Remineralization of Tooth Enamel [J].
Cochrane, N. J. ;
Cai, F. ;
Huq, N. L. ;
Burrow, M. F. ;
Reynolds, E. C. .
JOURNAL OF DENTAL RESEARCH, 2010, 89 (11) :1187-1197
[6]   The structural biology of the developing dental enamel matrix [J].
Fincham, AG ;
Moradian-Oldak, J ;
Simmer, JP .
JOURNAL OF STRUCTURAL BIOLOGY, 1999, 126 (03) :270-299
[7]  
GWINNETT AJ, 1992, OPER DENT, P10
[8]   Detection of caries and composite resin restorations by near-infrared Raman spectroscopy [J].
Hill, W ;
Petrou, V .
APPLIED SPECTROSCOPY, 1997, 51 (09) :1265-1268
[9]  
Ismail A I, 2004, J Dent Res, V83 Spec No C, pC56
[10]   Real-time global Raman imaging and optical manipulation of suspended carbon nanotubes [J].
Kaminska, Kate ;
Lefebvre, Jacques ;
Austing, D. Guy ;
Finnie, Paul .
PHYSICAL REVIEW B, 2006, 73 (23)