Joint Action Toxicity and Biochemical effects of Binary Mixtures of Forcados Light Crude Oil and Three Dispersants against Clarias gariepinus

被引：0

作者：

Sogbanmu, T. O. ^{[1
]}

Otitoloju, A. A. ^{[1
]}

机构：

[1] Univ Lagos, Ecotoxicol Unit, Dept Zool, Fac Sci, Lagos, Nigeria

来源：

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH | 2014年 / 8卷 / 02期

关键词：

Clarias gariepinus; Biochemical parameters; Forcados light crude oil; Dispersants; Joint action toxicity; GLUTATHIONE S-TRANSFERASES; OXIDATIVE STRESS; SUPEROXIDE-DISMUTASE; LIPID-PEROXIDATION; RESPONSES; TISSUE; FISH; ANTIOXIDANTS; POLLUTION; MUSSELS;

D O I：

暂无

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Laboratory-scale experiments were conducted to evaluate the joint action toxicity and biochemical effects of sublethal concentrations of Forcados light crude oil (FLCO) and three dispersants against Clarias gariepinus over a period of 28 days. The derived 96hrLC(50) values revealed that the dispersant, DS/TT/066 (0.03mL/L) was the most toxic, followed by dispersant, OC/TT/OSI (0.19mL/L), FLCO (5.06mL/L) and crystal clear oil dispersant (CCOD = 12.06mL/L) the least toxic when acting singly. Joint action toxicity evaluations of FLCO and dispersants showed that the interaction between FLCO : DS/TT/066 and FLCO : OC/TT/OSI was synergistic (synergistic ratio (SR) > I) with SR values of 10.5 and 3 respectively. However, for the mixture of FLCO : CCOD, the interaction was antagonistic (SR < I) with SR value of 0.97. The result of the biochemical effects study revealed that malondialdehyde (MDA) levels decreased significantly (P<0.05) in the exposed fishes, reduced gluthathione (GSH) and gluthathione-s-transferase (GST) activities increased significantly (P<0.05) in fishes exposed to FLCO : CCOD mixture alone while there was no significant difference (p>0.05) in superoxide dismutase (SOD) and catalase (CAT) activities in all the exposed fishes compared to control animals. The observed increase in GSH and GST levels in conjunction with a decrease in MDA concentration in the liver of test animals exposed to binary mixtures of FLCO and CCOD reveals the ability of the animals to overcome the effects of lipid peroxidation in this group. Further studies on the mechanism of toxicity of these dispersants in field and laboratory assays are recommended.

引用

页码：395 / 402

页数：8

共 39 条

[11]

FOURNIER D, 1992, J BIOL CHEM, V267, P1840

[12] Antioxidants in the black mussel (Mytilus galloprovincialis) as an indicator of black sea coastal pollution [J].

Gorinstein, S ;

Moncheva, S ;

Katrich, E ;

Toledo, F ;

Arancibia, P ;

Goshev, I ;

Trakhtenberg, S .

MARINE POLLUTION BULLETIN, 2003, 46 (10) :1317-1325

[13]

HABIG WH, 1974, J BIOL CHEM, V249, P7130

[14] A UNIFIED THEORY FOR QUANTAL RESPONSES TO MIXTURES OF DRUGS - NON-INTERACTIVE ACTION [J].

HEWLETT, PS ;

PLACKETT, RL .

BIOMETRICS, 1959, 15 (04) :591-610

[15] Oxidative stress in toxicology: Established mammalian and emerging piscine model systems [J].

Kelly, SA ;

Havrilla, CM ;

Brady, TC ;

Abramo, KH ;

Levin, ED .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1998, 106 (07) :375-384

[16] A piscine glutathione S-transferase which efficiently conjugates the end-products of lipid peroxidation [J].

Leaver, MJ ;

George, SG .

MARINE ENVIRONMENTAL RESEARCH, 1998, 46 (1-5) :71-74

[17] Characterization of an inducible isoform of the Cu/Zn superoxide dismutase in the blue mussel Mytilus edulis [J].

Manduzio, H ;

Monsinjon, T ;

Rocher, W ;

Leboulenger, F ;

Galap, C .

AQUATIC TOXICOLOGY, 2003, 64 (01) :73-83

[18]

Odiete W. O., 2003, J NIGERIA ENV SOC, V1, P340

[19] Tissue-specific oxidative stress responses in fish exposed to 2,4-D and azinphosmethyl [J].

Oruc, EO ;

Sevgiler, Y ;

Uner, N .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 2004, 137 (01) :43-51

[20] Crude oil plus dispersant: always a boon or bane? [J].

Otitoloju, AA .

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2005, 60 (02) :198-202

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