Freeze-fracture electron microscopy on nanostructures for drug & gene delivery

被引：0
作者：
Papahadjopoulos-Sternberg, B [1 ]
机构：
[1] NanoAnalyt Lab, San Francisco, CA 94118 USA
来源：
NANOTECH 2003, VOL 1 | 2003年
关键词：
electron microscopy; freeze-fracture technique; drug carrier; gene delivery; cell interaction;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The morphology of a variety of nanostructures suitable for drug and/or gene delivery such as liposomes, niosomes, cochleate cylinder, depofoam particles, and liposome/DNA complexes has been studied under biologically relevant conditions by using freeze-fracture electron microscopy. This study shows that freeze-fracture electron microscopy is not only a powerful technique to characterize drug/gene carrier but also the method of choice to study their fate related to the incubation milieu and drug/gene load, and during their interaction with cells on a nanometer resolution scale. Furthermore, parallel studies of the morphology and transfection activity help us to understand the fundamental difference between in vitro and in vivo transfection activity of cationic liposome/ DNA complexes as non-viral gene vectors.
引用
收藏
页码：40 / 43
页数：4
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