Characterisation of a novel mouse liver aldo-keto reductase AKR7A5

被引：41

作者：

Hinshelwood, A

McGarvie, G

Ellis, E

机构：

[1] Univ Strathclyde, Dept Pharmaceut Sci, Glasgow G1 1XW, Lanark, Scotland

[2] Bell Coll, Sch Sci & Technol, Hamilton ML3 OJB, Lanark, Scotland

[3] Univ Strathclyde, Dept Biosci, Glasgow G1 1XW, Lanark, Scotland

来源：

FEBS LETTERS | 2002年 / 523卷 / 1-3期

关键词：

aldo-keto reductase; succinic semialdehyde; mouse liver; carbonyl metabolism;

D O I：

10.1016/S0014-5793(02)02982-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have characterised a novel aldo-keto reductase (AKR7A5) from mouse liver that is 78% identical to rat aflatoxin dialdehyde reductase AKR7A1 and 89% identical to human succinic semialdehyde (SSA) reductase AKR7A2. AKR7A5 can reduce 2-carboxybenzaldehyde (2-CBA) and SSA as well as a range of aldehyde and diketone substrates. Western blots show that it is expressed in liver, kidney, testis and brain, and at lower levels in skeletal muscle, spleen heart and lung. The protein is not inducible in the liver by dietary ethoxyquin. Immunodepletion of AKR7A5 from liver extracts shows that it is one of the major liver 2-CBA reductases but that it is not the main SSA reductase in this tissue. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：213 / 218

页数：6

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