Antiviral and Immunomodulatory Effects of Norantea brasiliensis Choisy on Dengue Virus-2

被引：11
作者：
Fialho, Luciana Gomes [1 ]
da Silva, Vagner Pereira [2 ]
Nogueira Ignacio Reis, Sonia Regina [1 ]
Azeredo, Elzinandes Leal [1 ]
Coelho Kaplan, Maria Auxiliadora [3 ]
Figueiredo, Maria Raquel [2 ]
Kubelka, Claire Fernandes [1 ]
机构：
[1] Fiocruz MS, Inst Oswaldo Cruz, Lab Imunol Viral, Rio De Janeiro, Brazil
[2] Fiocruz MS, Inst Tecnol Farmacos, Lab Quim Prod Nat 3, Rio De Janeiro, Brazil
[3] Univ Fed Rio de Janeiro, Ctr Ciencias Saude, Inst Pesquisas Prod Nat, Rio de Janeiro, Brazil
来源：
INTERVIROLOGY | 2016年 / 59卷 / 04期
关键词：
Dengue; Norantea brasiliensis Choisy; Antiviral effect; Immunomodulation; ENDOTHELIAL-CELLS; UNCARIA-TOMENTOSA; IMMUNE-RESPONSES; HUMAN MONOCYTES; PLASMA LEAKAGE; NITRIC-OXIDE; INFECTION; DISEASE; EXPRESSION; CYTOKINES;
D O I：
10.1159/000455855
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Background/Aims: Severe dengue fever is a result of exacerbated immune responses and no specific treatments are available. We evaluated the antiviral and immunomodulatory effects of Norantea brasiliensis Choisy. Methods: Human adherent monocytes infected in vitro with dengue virus (DENV)-2 were incubated with the crude ethanol extract from leaves (NB1) or 3 derived fractions: dichloromethane (NB3), ethyl acetate (NB5), and butanolic (NB6) partitions. The antiviral and immunomodulatory activities were determined by intracellular detection of DENV antigen within monocytes and by secreted NS1 viral protein and cytokines. Results: The crude extract alone exhibited both antiviral activities (intracellular and secreted antigens) and all fractions derived from this extract modulated NS1 production. Regarding the immunomodulatory effect, among the secreted factors, TNF-alpha was inhibited by NB3 and NB6; IL-6 was inhibited by NB1, NB3, and NB6; IL-10 by NB1 and NB3; and IFN-alpha by NB6. The crude extract (NB1) presented the best antiviral effect, whereas the dichloromethane fraction (NB3) presented an immunomodulatory effect in the inflammatory and anti-inflammatory cytokines. Conclusion: During in vitro DENV infection, N. brasiliensis Choisy exerts both antiviral and immunomodulatory effects that are likely associated, considering that less viral load may lead to less immuno-stimulation. (C) 2017 S. Karger AG, Basel
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收藏
页码：217 / 227
页数：11
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