Extracellular Vesicles Derived from Kefir Grain Lactobacillus Ameliorate Intestinal Inflammation via Regulation of Proinflammatory Pathway and Tight Junction Integrity

被引：60

作者：

Kang, Eun Ae ^{[1
,2
]}

Choi, Hye-In ^{[3
]}

Hong, Seung Wook ^{[4
,5
]}

Kang, Seokwoo ^{[3
]}

Jegal, Hyeon-Young ^{[3
]}

Choi, Eun Wook ^{[3
]}

Park, Byung-Soon ^{[3
,6
]}

Kim, Joo Sung ^{[4
,5
]}

机构：

[1] Yonsei Univ, Coll Med, Dept Internal Med, Seoul 03722, South Korea

[2] Yonsei Univ, Coll Med, Inst Gastroenterol, Seoul 03722, South Korea

[3] Prostem Res Inst, Seoul 04778, South Korea

[4] Seoul Natl Univ, Dept Internal Med, Coll Med, Seoul 03080, South Korea

[5] Seoul Natl Univ, Liver Res Inst, Coll Med, Seoul 03080, South Korea

[6] Cellpk Clin, Seoul 06029, South Korea

来源：

BIOMEDICINES | 2020年 / 8卷 / 11期

关键词：

extracellular vesicle; Lactobacillus; experimental colitis; tight junction; NF-kappa B; NECROSIS-FACTOR-ALPHA; DEXTRAN SULFATE SODIUM; CYTOKINE PRODUCTION; EPITHELIAL BARRIER; BOWEL-DISEASE; BACTERIA; EXPRESSION; MECHANISM; PATHOGENESIS; INHIBITORS;

D O I：

10.3390/biomedicines8110522

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to demonstrate the anti-inflammatory effect of Lactobacillus kefirgranum PRCC-1301-derived extracellular vesicles (PRCC-1301 EVs) on intestinal inflammation and intestinal barrier function. Human intestinal epithelial cells (IECs) Caco-2 were treated with PRCC-1301 EVs and then stimulated with dextran sulfate sodium (DSS). Real-time RT-PCR revealed that PRCC-1301 EVs inhibited the expression of pro-inflammatory cytokines in Caco-2 cells. PRCC-1301 EVs enhanced intestinal barrier function by maintaining intestinal cell integrity and the tight junction. Loss of Zo-1, claudin-1, and occludin in Caco-2 cells and the colitis tissues was recovered after PRCC-1301 EVs treatment, as evidenced by immunofluorescence analysis. Acute murine colitis was induced using 4% DSS and chronic colitis was generated in piroxicam-treated IL-10(-/-) mice. PRCC-1301 EVs attenuated body weight loss, colon shortening, and histological damage in acute and chronic colitis models in mice. Immunohistochemistry revealed that phosphorylated NF-kappa B p65 and I kappa B alpha were reduced in the colon tissue sections treated with PRCC-1301 EVs. Our results suggest that PRCC-1301 EVs may have an anti-inflammatory effect on colitis by inhibiting the NF-kappa B pathway and improving intestinal barrier function.

引用

页码：1 / 11

页数：11

共 43 条

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