Simple Isolation of Single Cell: Thin Glass Microfluidic Device for Observation of Isolated Single Euglena gracilis Cells

被引：8

作者：

Ota, Nobutoshi ^{[1
]}

Yalikun, Yaxiaer ^{[1
,2
]}

Tanaka, Nobuyuki ^{[1
]}

Shen, Yigang ^{[1
]}

Aishan, Yusufu ^{[1
,3
]}

Nagahama, Yuki ^{[3
]}

Oikawa, Minoru ^{[3
]}

Tanaka, Yo ^{[1
]}

机构：

[1] RIKEN, Ctr Biosyst Dynam Res, 1-3 Yamadaoka, Suita, Osaka 5650871, Japan

[2] Nara Inst Sci & Technol, Div Mat Sci, Nara 6300192, Japan

[3] Chiba Univ, Grad Sch Engn, Dept Engn, Inage Ku, 1-33 Yayoi, Chiba, Chiba 2638522, Japan

来源：

ANALYTICAL SCIENCES | 2019年 / 35卷 / 05期

关键词：

Glass microfluidics; single cell isolation; machine vision; QUANTUM-DOT; FUTURE; CULTURE; CHIP;

D O I：

10.2116/analsci.18P568

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Single cell analysis has gained attention as a means to investigate the heterogeneity of cells and amplify a cell with desired characteristics. However, obtaining a single cell from a large number of cells remains difficult because preparation of single-cell samples relies on conventional techniques such as pipetting that are labor intensive. In this study, we developed a system combining a 0.6-mm thin glass microfluidic device and machine vision approach to isolate single Euglena gracilis cells, as a model of microorganism with mobility, in a small/thin glass chamber. A single E. gracilis cell in a chamber was cultured for 4 days to monitor its multiplication. With this system, we successfully simplified preparation of single cells of interest and determined that it is possible to combine it with other analytical techniques to observe single cells continuously.

引用

页码：577 / 583

页数：7

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