Mesoporous silica nanoparticles for enhancing the delivery efficiency of immunostimulatory DNA drugs

被引：46
作者：
Tao, Cuilian [1 ]
Zhu, Yufang [2 ]
Xu, Yi [1 ]
Zhu, Min [2 ]
Morita, Hiromi [3 ]
Hanagata, Nobutaka [3 ]
机构：
[1] Univ Shanghai Sci & Technol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China
[2] Univ Shanghai Sci & Technol, Sch Mat Sci & Engn, Shanghai 200093, Peoples R China
[3] Natl Inst Mat Sci, Nanotechnol Innovat Stn, Tsukuba, Ibaraki 3050047, Japan
来源：
DALTON TRANSACTIONS | 2014年 / 43卷 / 13期
基金：
中国国家自然科学基金;
关键词：
CONTROLLED-RELEASE; GENE-EXPRESSION; CO-DELIVERY; IN-VIVO; SIRNA; TRANSFECTION; CELLS; INTERFERON; SYSTEM; SENSOR;
D O I：
10.1039/c3dt53433b
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
We developed a potential immunostimulatory double-stranded DNA (dsDNA) delivery system by the binding of dsDNA to amino-modified mesoporous silica nanoparticles (MSNs) to form MSN-NH2/dsDNA complexes. Serum stability, in vitro cytotoxicity, cell uptake, and type I interferon-alpha (IFN-alpha) induction of MSN-NH2/dsDNA complexes were evaluated. The results showed that MSN-NH2 nanoparticles had no cytotoxicity to Raw 264.7 cells, and MSN-NH2/dsDNA complexes enhanced the serum stability of dsDNA due to the protection by nanoparticles and exhibited a high efficiency of cell uptake due to a small particle size and excellent dispersity. Most importantly, MSN-NH2/dsDNA complexes significantly enhanced the level of IFN-alpha induction, triggered by cytosolic DNA sensor proteins. Therefore, binding of immunostimulatory DNA to MSNs would play a promising role for enhancing the delivery efficiency of immunostimulatory DNA drugs.
引用
收藏
页码：5142 / 5150
页数：9
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