Mesoporous silica nanoparticles for enhancing the delivery efficiency of immunostimulatory DNA drugs

被引：46
作者：
Tao, Cuilian [1 ]
Zhu, Yufang [2 ]
Xu, Yi [1 ]
Zhu, Min [2 ]
Morita, Hiromi [3 ]
Hanagata, Nobutaka [3 ]
机构：
[1] Univ Shanghai Sci & Technol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China
[2] Univ Shanghai Sci & Technol, Sch Mat Sci & Engn, Shanghai 200093, Peoples R China
[3] Natl Inst Mat Sci, Nanotechnol Innovat Stn, Tsukuba, Ibaraki 3050047, Japan
来源：
DALTON TRANSACTIONS | 2014年 / 43卷 / 13期
基金：
中国国家自然科学基金;
关键词：
CONTROLLED-RELEASE; GENE-EXPRESSION; CO-DELIVERY; IN-VIVO; SIRNA; TRANSFECTION; CELLS; INTERFERON; SYSTEM; SENSOR;
D O I：
10.1039/c3dt53433b
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
We developed a potential immunostimulatory double-stranded DNA (dsDNA) delivery system by the binding of dsDNA to amino-modified mesoporous silica nanoparticles (MSNs) to form MSN-NH2/dsDNA complexes. Serum stability, in vitro cytotoxicity, cell uptake, and type I interferon-alpha (IFN-alpha) induction of MSN-NH2/dsDNA complexes were evaluated. The results showed that MSN-NH2 nanoparticles had no cytotoxicity to Raw 264.7 cells, and MSN-NH2/dsDNA complexes enhanced the serum stability of dsDNA due to the protection by nanoparticles and exhibited a high efficiency of cell uptake due to a small particle size and excellent dispersity. Most importantly, MSN-NH2/dsDNA complexes significantly enhanced the level of IFN-alpha induction, triggered by cytosolic DNA sensor proteins. Therefore, binding of immunostimulatory DNA to MSNs would play a promising role for enhancing the delivery efficiency of immunostimulatory DNA drugs.
引用
收藏
页码：5142 / 5150
页数：9
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共 47 条
  • [1] RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate
    Ablasser, Andrea
    Bauernfeind, Franz
    Hartmann, Gunther
    Latz, Eicke
    Fitzgerald, Katherine A.
    Hornung, Veit
    [J]. NATURE IMMUNOLOGY, 2009, 10 (10) : 1065 - U40
  • [2] Intracellular localization of lipoplexed siRNA in vascular endothelial cells of different mouse tissues
    Aleku, Manuela
    Fisch, Gerald
    Moepert, Kristin
    Keil, Oliver
    Arnold, Wolfgang
    Kaufmann, Joerg
    Santel, Ansgar
    [J]. MICROVASCULAR RESEARCH, 2008, 76 (01) : 31 - 41
  • [3] Co-delivery of Doxorubicin and Bcl-2 siRNA by Mesoporous Silica Nanoparticles Enhances the Efficacy of Chemotherapy in Multidrug-Resistant Cancer Cells
    Chen, Alex M.
    Zhang, Min
    Wei, Dongguang
    Stueber, Dirk
    Taratula, Oleh
    Minko, Tamara
    He, Huixin
    [J]. SMALL, 2009, 5 (23) : 2673 - 2677
  • [4] Magnetically-enabled and MR-monitored selective brain tumor protein delivery in rats via magnetic nanocarriers
    Chertok, Beata
    David, Allan E.
    Yang, Victor C.
    [J]. BIOMATERIALS, 2011, 32 (26) : 6245 - 6253
  • [5] Mesoporous silica nanoparticles for the design of smart delivery nanodevices
    Colilla, Montserrat
    Gonzalez, Blanca
    Vallet-Regi, Maria
    [J]. BIOMATERIALS SCIENCE, 2013, 1 (02) : 114 - 134
  • [6] Mesoporous silica-supported lipid bilayers (protocells) for DNA cargo delivery to the spinal cord
    Dengler, Ellen C.
    Liu, Juewen
    Kerwin, Audra
    Torres, Sergio
    Olcott, Clara M.
    Bowman, Brandi N.
    Armijo, Leisha
    Gentry, Katherine
    Wilkerson, Jenny
    Wallace, James
    Jiang, Xingmao
    Carnes, Eric C.
    Brinker, C. Jeffrey
    Milligan, Erin D.
    [J]. JOURNAL OF CONTROLLED RELEASE, 2013, 168 (02) : 209 - 224
  • [7] Differential immune activation following encapsulation of immunostimulatory CpG oligodeoxynucleotide in nanoliposomes
    Erikci, Erdem
    Gursel, Mayda
    Gursel, Ihsan
    [J]. BIOMATERIALS, 2011, 32 (06) : 1715 - 1723
  • [8] Graphene based gene transfection
    Feng, Liangzhu
    Zhang, Shuai
    Liu, Zhuang
    [J]. NANOSCALE, 2011, 3 (03) : 1252 - 1257
  • [9] Effect of size on the cellular endocytosis and controlled release of mesoporous silica nanoparticles for intracellular delivery
    Gan, Qi
    Dai, Danwei
    Yuan, Yuan
    Qian, Jiangchao
    Sha, Sha
    Shi, Jianlin
    Liu, Changsheng
    [J]. BIOMEDICAL MICRODEVICES, 2012, 14 (02) : 259 - 270
  • [10] Structure-dependent immunostimulatory effect of CpG oligodeoxynucleotides and their delivery system
    Hanagata, Nobutaka
    [J]. INTERNATIONAL JOURNAL OF NANOMEDICINE, 2012, 7 : 2181 - 2195
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