Microbial recycling of glycerol to biodiesel

被引：13

作者：

Yang, Liu ^{[1
,2
]}

Zhu, Zhi ^{[1
]}

Wang, Weihua ^{[1
]}

Lu, Xuefeng ^{[1
]}

机构：

[1] Chinese Acad Sci, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 150卷

关键词：

Biodiesel; Glycerol; Escherichia coli; Genetic engineering; Fermentation; ESCHERICHIA-COLI; CRUDE GLYCEROL; CHEMICALS; BIOFUELS; CHALLENGES; ETHANOL; BIOMASS; FUELS;

D O I：

10.1016/j.biortech.2013.09.073

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The sustainable supply of lipids is the bottleneck for current biodiesel production. Here microbial recycling of glycerol, byproduct of biodiesel production to biodiesel in engineered Escherichia coli strains was reported. The KC3 strain with capability of producing fatty acid ethyl esters (FAEEs) from glucose was used as a starting strain to optimize fermentation conditions when using glycerol as sole carbon source. The YL15 strain overexpressing double copies of atfA gene displayed 1.7-fold increase of FAEE productivity compared to the KC3 strain. The titer of FAEE in YL15 strain reached to 813 mg L-1 in minimum medium using glycerol as sole carbon source under optimized fermentation conditions. The titer of glycerol-based FAEE production can be significantly increased by both genetic modifications and fermentation optimization. Microbial recycling of glycerol to biodiesel expands carbon sources for biodiesel production. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：1 / 8

页数：8

共 28 条

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