iRoot BP Plus promotes osteo/odontogenic differentiation of bone marrow mesenchymal stem cells via MAPK pathways and autophagy

被引：55
作者：
Lu, Jiamin [1 ]
Li, Zehan [1 ]
Wu, Xiao [1 ]
Chen, Yan [3 ]
Yan, Ming [1 ,2 ]
Ge, Xingyun [1 ]
Yu, Jinhua [1 ,2 ]
机构：
[1] Nanjing Med Univ, Inst Stomatol, Key Lab Oral Dis Jiangsu Prov, 136 Hanzhong Rd, Nanjing 210029, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Sch Stomatol, Endodont Dept, 136 Hanzhong Rd, Nanjing 210029, Jiangsu, Peoples R China
[3] Nanjing Univ, Sch Med, Nanjing Stomatol Hosp, 30 Zhongyang Rd, Nanjing 210008, Jiangsu, Peoples R China
来源：
STEM CELL RESEARCH & THERAPY | 2019年 / 10卷 / 1期
基金：
中国国家自然科学基金;
关键词：
iRoot BP Plus; BMMSCs; Osteo; odontogenic differentiation; Autophagy; MAPK pathways; MINERAL TRIOXIDE AGGREGATE; OSTEOGENIC DIFFERENTIATION; IN-VITRO; STROMAL CELLS; KAPPA-B; PULP; CAPACITY; MIGRATION; CALCIUM; RELEASE;
D O I：
10.1186/s13287-019-1345-3
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
BackgroundiRoot BP Plus is a novel bioceramic endodontic material. Recently, it has been considered as an alternative to MTA which is the most popular scaffold cover during regenerative endodontic therapy. This study aimed to evaluate the effects of iRoot BP Plus on the osteo/odontogenic capacity of bone marrow mesenchymal stem cells (BMMSCs), including the underlying mechanisms.MethodsBMMSCs were collected by a whole marrow method and treated with iRoot BP Plus-conditioned medium (BP-CM). The proliferation ability was evaluated by cell counting kit 8 and flow cytometry. Complete medium was used as a blank control, and 2mg/ml MTA-conditioned medium wasserved as a positive control. Alkaline phosphatase (ALP) activity assay, ALP staining, western blot, real-time RT-PCR, Alizarin Red S staining, and immunofluorescence staining were performed to explore the osteo/odontogenic potential and the involvement of MAPK pathways. Besides, autophagy was investigated by western blot, immunofluorescence staining, and transmission electron microscopy.Results
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页数：14
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