Development and Characterization of EST-SSR Markers for Tephroseris furusei (Kitam.) B. Nord. (Asteraceae) and Cross-species Amplification

被引：0

作者：

Mitsui, Yuki ^{[1
]}

Sato, Chiyoshi ^{[2
]}

Chiba, Satoshi ^{[3
]}

Tsuchiyama, Tatsuya ^{[1
]}

Fuchino, Kento ^{[1
]}

Asano, Yuki ^{[1
]}

Tanaka, Keisuke ^{[4
]}

Miyamoto, Futoshi ^{[1
]}

机构：

[1] Tokyo Univ Agr, Atsugi, Kanagawa 2430034, Japan

[2] Kumamoto Plant Res Stn, Kita Ku, Kumamoto 8615517, Japan

[3] Omachi Alpine Museum, Omachi, Nagano 3980002, Japan

[4] Tokyo Univ Agr, NODAI Genome Res Ctr, Setagaya Ku, 1-1-1 Sakuragaoka, Tokyo 1568502, Japan

来源：

ACTA PHYTOTAXONOMICA ET GEOBOTANICA | 2022年 / 73卷 / 01期

关键词：

conservation genetics; endangered plants; EST-SSR; fixation index; genetic diversity; microsatellite; Tephroseris; DELIMITATION; PHYLOGENY;

D O I：

10.18942/apg.202117

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Twelve polymorphic EST-SSR markers were developed and characterized for Tephroseris furusei (Kitam.) B. Nord., an endangered species endemic to limestone habitats in central Japan, and related species. In the two T. furusei populations, the total numbers of alleles for each locus ranged from 5 to 16 (average = 8.75). The observed (HO) and expected (HE) heterozygosities ranged from 0.33 to 0.76 (average = 0.58) and 0.50 to 0.89 (average = 0.75), respectively. The inbreeding coefficient (fixation index, FIS) ranged from 0.14 to 0.50 (average = 0.24) and five loci deviated significantly from Hardy-Weinberg equilibrium. The versatility of the markers was evaluated for three congenerics: T. flammea (Turcz. ex DC.) Holub subsp. flammea, T. integrifolia (L.) Holub subsp. kirilowii (Turcz. ex DC.), and T. pierotii (Miq.) Holub. All of the EST-SSR markers were transferrable among them.

引用

页码：87 / 92

页数：6

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