Isolation, purification and immunobiological activity of a new water-soluble bee pollen polysaccharide from Crataegus pinnatifida Bge.

被引：98

作者：

Li, Fei ^{[1
]}

Yuan, Qipeng ^{[1
]}

Rashid, Farzana ^{[1
]}

机构：

[1] Beijing Univ Chem Technol, Sch Life Sci, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2009年 / 78卷 / 01期

关键词：

Crataegus pinnatifida Bge; Polysaccharide; Monosaccharide component; Phagocytic function; Splenocytes proliferation; NK cells; CELL-WALL POLYSACCHARIDES; ANTITUMOR-ACTIVITY; ALDITOL ACETATES; IN-VITRO; CHROMATOGRAPHY; MYCELIUM; CULTURE; SPORES; PLANT; L;

D O I：

10.1016/j.carbpol.2009.04.005

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A novel water-soluble polysaccharide was obtained from bee pollen of Crataegus pinnatifida Bge. Two fractions of this polysaccharide, CPP-1 and CPP-2, were first extracted by hot-water and purified. The average molecular weight of CPP-1 and CPP-2 were approximately 3.7 x 10(5) Da and 7.8 x 10(4) Da, and their chemical structures were studied by gas chromatography (GC), Fourier transform-infrared (FT-IR) spectroscopy, methylation analysis. We evaluated the effects of CPP-1 and CPP-2 on the basis of phagocytosis of macrophage assay, natural killer cells cytotoxicity assay and spleen lymphocyte proliferation assay. The results showed CPP-1 and CPP-2 significantly induced phagocytic rates and phagocytic indexes by peritoneal macrophages. Moreover, these two fractions caused a significant stimulation of rat spleen cell proliferation. At 50 mu g/mL, CPP-2 activated NK cells more significantly than CPP-1. These findings suggest that they should be explored as a novel potential immunostimulants. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：80 / 88

页数：9

共 37 条

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