Antibody-based fluorescence detection of kinase activity on a peptide array

被引：100

作者：

Lesaicherre, ML

Uttamchandani, M

Chen, GYJ

Yao, SQ

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2002年 / 12卷 / 16期

关键词：

D O I：

10.1016/S0960-894X(02)00378-5

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Peptide-based microarrays allow for high-throughput identification of protein kinase substrates. However, current methods of detecting kinase activity require the use of radioisotopes. We have developed a novel fluorescence-based approach for quantitative detection of peptide phosphorylation on chip using fluorescently-labeled anti-phosphoserine and anti-phosphotyrosine antibodies. This method is sensitive, specific and extremely fast, presenting obvious advantages and may find wider uses in high-throughput kinase screenings. (C) 2002 Elsevier Science Ltd. All rights reserved.

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页码：2085 / 2088

页数：4

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