LAMP real-time turbidity detection for fowl adenovirus

被引：22
|
作者：
Yuan, Xiao-Yuan [1 ,2 ]
Wang, You-Ling [1 ,2 ]
Meng, Kai [1 ,2 ]
Zhang, Yu-Xia [1 ,2 ]
Xu, Huai-Ying [1 ,2 ]
Ai, Wu [1 ,2 ]
机构：
[1] Shandong Acad Agr Sci, Poultry Inst, Jinan, Shandong, Peoples R China
[2] Poultry Breeding Engn Technol Ctr Shandong Prov, Shandong Prov Key Lab Poultry Dis Diag & Immunolo, Jinan, Shandong, Peoples R China
来源：
BMC VETERINARY RESEARCH | 2019年 / 15卷 / 1期
关键词：
Adenovirus; LAMP; Real-time turbidity; Visual detection; VIRUS; ASSAY;
D O I：
10.1186/s12917-019-2015-5
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
BackgroundFowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established.ResultsThe established assay was specific to FAdV-4, and the lower limit of detection was 75 copies/mu L of extracted DNA. The positive detection rate for the suspected tissue samples was 33.3% (14/42), which was consistent with that of the real-time PCR.ConclusionThe proposed LAMP method can quickly and accurately detect prevalent FAdV via real-time turbidity assay, thereby providing a diagnostic platform for the prevention and control of the FAdV disease.
引用
收藏
页数：4
相关论文
共 50 条
  • [41] Real-time contamination detection
    Precision Cleaning, 2000, 8 (02):
  • [42] Reading Detection in Real-time
    Kelton, Conor
    Wei, Zijun
    Ahn, Seoyoung
    Balasubramanian, Aruna
    Das, Samir R.
    Samaras, Dimitris
    Zelinsky, Gregory
    ETRA 2019: 2019 ACM SYMPOSIUM ON EYE TRACKING RESEARCH & APPLICATIONS, 2019,
  • [43] Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric population
    De Vos, N.
    Vankeerberghen, A.
    Vaeyens, F.
    Van Vaerenbergh, K.
    Boel, A.
    De Beenhouwer, H.
    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2009, 28 (11) : 1305 - 1310
  • [44] Real-time turbidimetry of LAMP reaction for quantifying template DNA
    Mori, Y
    Kitao, M
    Tomita, N
    Notomi, T
    JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2004, 59 (02): : 145 - 157
  • [45] Evaluation of a real-time multiplex PCR assay for the detection of enteric rotavirus, adenovirus, astrovirus and sapovirus
    Fleming, A. -M.
    Guiver, M.
    JOURNAL OF CLINICAL VIROLOGY, 2009, 46 : S40 - S40
  • [46] Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric population
    N. De Vos
    A. Vankeerberghen
    F. Vaeyens
    K. Van Vaerenbergh
    A. Boel
    H. De Beenhouwer
    European Journal of Clinical Microbiology & Infectious Diseases, 2009, 28 : 1305 - 1310
  • [47] Multiwavelength Photosensor for On-Chip Real-Time Monitoring of Fluorescence and Turbidity
    Maruyama, Yuki
    Ishida, Makoto
    Sawada, Kazuaki
    JAPANESE JOURNAL OF APPLIED PHYSICS, 2009, 48 (06)
  • [48] A photonic sensor system for real-time monitoring of turbidity changes in aquaculture
    Tolon, Mustafa Tolga
    Tokac, Adnan
    Kostak, Enis N.
    Strehse, Christoph
    NORTH AMERICAN JOURNAL OF AQUACULTURE, 2024, 86 (04) : 424 - 432
  • [49] A novel voltammetric approach for real-time electrochemical detection of targeted nucleic acid sequences using LAMP
    Hashimoto, Koji
    Inada, Mika
    Ito, Keiko
    ANALYTICAL BIOCHEMISTRY, 2017, 539 : 113 - 117
  • [50] Real-time LAMP detection of Cucurbit leaf crumple virus and comparison with PCR-based techniques
    Waliullah, S.
    Ling, K. S.
    Cieniewicz, E.
    Oliver, J. E.
    Ji, P.
    Ali, E.
    PHYTOPATHOLOGY, 2020, 110 (12) : 99 - 100
12345