LAMP real-time turbidity detection for fowl adenovirus

被引：22
|
作者：
Yuan, Xiao-Yuan [1 ,2 ]
Wang, You-Ling [1 ,2 ]
Meng, Kai [1 ,2 ]
Zhang, Yu-Xia [1 ,2 ]
Xu, Huai-Ying [1 ,2 ]
Ai, Wu [1 ,2 ]
机构：
[1] Shandong Acad Agr Sci, Poultry Inst, Jinan, Shandong, Peoples R China
[2] Poultry Breeding Engn Technol Ctr Shandong Prov, Shandong Prov Key Lab Poultry Dis Diag & Immunolo, Jinan, Shandong, Peoples R China
来源：
BMC VETERINARY RESEARCH | 2019年 / 15卷 / 1期
关键词：
Adenovirus; LAMP; Real-time turbidity; Visual detection; VIRUS; ASSAY;
D O I：
10.1186/s12917-019-2015-5
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
BackgroundFowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established.ResultsThe established assay was specific to FAdV-4, and the lower limit of detection was 75 copies/mu L of extracted DNA. The positive detection rate for the suspected tissue samples was 33.3% (14/42), which was consistent with that of the real-time PCR.ConclusionThe proposed LAMP method can quickly and accurately detect prevalent FAdV via real-time turbidity assay, thereby providing a diagnostic platform for the prevention and control of the FAdV disease.
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页数：4
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