Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing

被引：34

作者：

Douglas, Timothy E. L. ^{[1
,2
,3
]}

Dziadek, Michal ^{[4
]}

Gorodzha, Svetlana ^{[5
]}

Liskova, Jana ^{[6
]}

Brackman, Gilles ^{[7
]}

Vanhoorne, Valerie ^{[8
]}

Vervaet, Chris ^{[8
]}

Balcaen, Lieve ^{[9
]}

Garcia, Maria del Rosario Florez ^{[9
]}

Boccaccini, Aldo R. ^{[10
]}

Weinhardt, Venera ^{[11
,12
,13
]}

Baumbach, Tilo ^{[12
,13
]}

Vanhaecke, Frank ^{[9
]}

Coenye, Tom ^{[7
]}

Bacakova, Lucie ^{[6
]}

Surmeneva, Maria A. ^{[5
]}

Surmenev, Roman A. ^{[5
]}

Cholewa-Kowalska, Katarzyna ^{[4
]}

Skirtach, Andre G. ^{[1
,14
]}

机构：

[1] Univ Ghent, Dept Mol Biotechol, Coupure Links 653, B-9000 Ghent, Belgium

[2] Univ Lancaster, Engn Dept, Lancaster, England

[3] Univ Lancaster, MSI, Lancaster, England

[4] AGH Univ Sci & Technol, Dept Glass Technol & Amorphous Coatings, Krakow, Poland

[5] Natl Res Tomsk Polytech Univ, Dept Theoret & Expt Phys, Tomsk, Russia

[6] Czech Acad Sci, Inst Physiol, Dept Biomat & Tissue Engn, Prague, Czech Republic

[7] Univ Ghent, Fac Pharmaceut Sci, Dept Pharmaceut Anal, Lab Pharmaceut Microbiol, Ghent, Belgium

[8] Univ Ghent, Fac Pharmaceut Sci, Dept Pharmaceut, Lab Pharmaceut Technol, Ghent, Belgium

[9] Univ Ghent, Dept Analyt Chem, Ghent, Belgium

[10] Univ Erlangen Nurnberg, Inst Biomat, Dept Mat Sci & Engn, Erlangen, Germany

[11] Heidelberg Univ, Ctr Organismal Studies, Heidelberg, Germany

[12] Karlsruhe Inst Technol, Lab Applicat Synchrotron Radiat, Karlsruhe, Germany

[13] Karlsruhe Inst Technol, Inst Photon Sci & Synchrotron Radiat, Karlsruhe, Germany

[14] Univ Ghent, Ctr Nano & Biophoton, Ghent, Belgium

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2018年 / 12卷 / 06期

关键词：

MINERALIZATION; PHOSPHATE; DIFFERENTIATION; SCAFFOLDS; BIOGLASS; PROLIFERATION; MAGNESIUM; SODIUM; CELLS; FILMS;

D O I：

10.1002/term.2654

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mineralization of hydrogel biomaterials is desirable to improve their suitability as materials for bone regeneration. In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles. This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass. Gelation occurred within 20min. Composites containing the standard 45S5 bioactive glass preparation were markedly less stiff. X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8m, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels. The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5). A2, A2Zn, and A2Sr bioactive glass particles were more homogeneously dispersed in GG hydrogels than 45S5. Composites containing all four bioactive glass preparations exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5. All composites underwent mineralization with calcium-deficient hydroxyapatite upon incubation in simulated body fluid. The extent of mineralization appeared to be greatest for composites containing A2Zn5 and 45S5. The results underline the importance of the choice of bioactive glass when preparing injectable, self-gelling composites.

引用

页码：1313 / 1326

页数：14

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