Phenolic compounds modulation in β-farnesene fed-batch fermentation using sugarcane syrup as feedstock

被引：14

作者：

Carvalho, Luis Carlos ^{[1
,2
]}

Oliveira, Ana L. S. ^{[2
]}

Carsanba, Erdem ^{[1
,2
]}

Pintado, Manuela ^{[2
]}

Oliveira, Carla ^{[2
]}

机构：

[1] Unipessoal Lda, Amyris BioProd Portugal, Rua Diogo Botelho 1327, P-4169005 Porto, Portugal

[2] Univ Catolica Portuguesa, CBQF Ctr Biotecnol & Quim Fina, Escola Super Biotecnol, Lab Associado, Rua Diogo Botelho 1327, P-4169005 Porto, Portugal

来源：

INDUSTRIAL CROPS AND PRODUCTS | 2022年 / 188卷

关键词：

Sugarcane syrup; Phenolic compounds; Saccharomyces cerevisiae; beta-farnesene; Fed-batch fermentation; RADICAL SCAVENGING ACTIVITY; LACTIC-ACID FERMENTATION; ANTIOXIDANT ACTIVITY; INHIBITORS; PHYTOCHEMICALS; CONSTITUENTS; DERIVATIVES; MECHANISM;

D O I：

10.1016/j.indcrop.2022.115721

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Sugarcane syrup is the feedstock used in the industrial production of beta-farnesene through Saccharomyces cerevisiae fermentation. However, it contains phenolic compounds, which may interfere with yeast performance. The aim of this work was to modulate the transfer of phenolic compounds from sugarcane syrup to the broth, throughout 13 days of beta-farnesene fed-batch fermentation in 2-L bioreactors. This was accomplished by applying two mathematical calculations: a mass balance and the Weibull kinetic model. The concentrations of most phenolic compounds increased until day 3 of fermentation and then remained constant until the end of the process. However, quantities of hydroxybenzaldehyde, protocatechuic, caffeic, ferulic and p-coumaric acids, decreased after day 2. The Weibull model exhibited better fit to the data, with an R-2 of 0.85 or higher. This work provides for the first time a model describing phenolic accumulation in beta-farnesene fermentation, which can be a valuable tool to apply in similar processes.

引用

页数：10

共 49 条

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